楊智欽2006-07-262018-07-112006-07-262018-07-112004http://ntur.lib.ntu.edu.tw//handle/246246/23635人類幽門螺旋桿菌 (Helicobacter pylori) 是一種常見的感染，且與胃炎，胃潰瘍 及胃部的癌症有密切的關係。幽門螺旋桿菌所引起的致病機轉目前仍不清楚。由 於此種感染普遍存在於正常及胃部有症狀的病人中，因此其導致不同程度疾病的 致病機轉可能與宿主因子以及Hp 菌株本身毒性因子兩者都有相關。我們之前也 發現一個H. pylori 菌株蛋白和胃淋巴瘤以及另一個菌株蛋白和胃癌可能相關而 分別發表於文獻中。最近我們研究發現使用二維電冰法來分離菌株蛋白質可以較 之前使用的1-D SDS-PAGE 有更好的解析能力，足以找出更多具有重要性的因 子。為了更進一步了解H. pylori 感染宿主的致病機轉，我們將自門診內視鏡檢 中選取診斷為Hp 陽性之胃部疾病(胃炎、消化性潰瘍、胃癌)為對象，同時對胃 部各夾取黏膜檢體送組織切片判定疾病類型且培養H. pylori 。將H. pylori 在厭氧 的狀態下進行液體培養，然後萃取分泌性蛋白質。將萃取的蛋白質用二維電泳法 將蛋白質分離開來，再用銀染色法染色，最後分析不同疾病的H. pylori 蛋白質, 再以液相層析—串聯式質譜儀做這些蛋白質樣本的身份鑑定分析，找出可能產生 不同臨床疾病有關的特異性菌株蛋白。結果我們發現：(1)菌體蛋白質：由十二 指腸潰瘍患者的幽門螺旋桿菌之二維電泳分析圖發現有3 種蛋白質為十二指腸 潰瘍患者的特異性蛋白質而其它種類病(胃潰瘍、胃炎、胃癌)則沒有。2 種蛋白 質為胃炎患者的特異性蛋白質。2 種蛋白質為胃癌患者的特異性蛋白質。(2)分泌 性蛋白質：由胃炎患者的幽門螺旋桿菌之二維電泳分析圖發現2 種蛋白質為胃炎 患者的特異性蛋白質；1 種蛋白質為胃癌患者的特異性蛋白質。Recent studies strongly suggest that chronic infection with H. pylori is tightly associated with chronic gastritis, peptic ulcer, and gastric carcinoma. However, only a part of infected people develop signs and symptoms of gastric pathology. Thus, both host and microbial factors may lead to different outcomes of infection. Perhaps the greatest concern with regard to infection with H. pylori is the increased risk for the development of gastric cancers in adulthood. This is particularly relevant because infection dating from childhood appears to enhance the risk of carcinogenesis. Recently, in a continuous epidemiological study for the H. pylori infection in the adolescence and children, we have found a steady proportion of these young individuals year by year having the ability to produce spontaneous clearance of the H. pylori infection. Meanwhile, in another studies, we also have identified two candidate proteins, one from the patients with gastric cancer, the other from the patients with gastric MALToma. In a pilot study, however, we have found many protein bands which demonstrated on a 1-D SDS-PAGE can be separated more clearly to several protein spots by 2-D gel electrophoresis plus immunoblotting detection. In this project, using technology of proteomics, we have found 1) whole-cell proteins: Three protein spots on the 2-DE gel were found in the H. pylori isolated from the patients with duodenal ulcer. These 3 protein spots were not found in the strains related to the patients with gastric ulcer and gastric cancer. Two protein spots on the 2-DE gel were specifically associated with chronic gastritis only. There were 2 protein spots on the 2-DE gel of H. pylori isolated from the patients with gastric cancer. These 2 protein spots were not noted in the strains related to the patients with other condition. 2) Secreted proteins: Two proteins spots were found from the H. pylori strains specifically related to the patients with chronic gastritis only. Meanwhile, there were one protein spot on the 2-DE gel of H. pylori isolated from the patients with gastric cancer. This protein spot was not noted in the strain associated with other disease patterns. This study will provide further insight not only in understanding of pathogenic mechanism in H. pylori infection, but also the direction of developing new therapies or vaccines.application/pdf504274 bytesapplication/pdfzh-TW國立臺灣大學醫學院內科幽門螺旋桿菌小鼠動物模式主要抗原Helicobacter pylorimajor antigenmouse animal model[SDGs]SDG3reporthttp://ntur.lib.ntu.edu.tw/bitstream/246246/23635/1/922314B002142.pdf