Huang C.-H.Lin Y.-C.TONG-RONG JAN2020-01-032020-01-0320171756-4646https://scholars.lib.ntu.edu.tw/handle/123456789/444595The effect of Lactobacillus reuteri against food allergy was investigated in ovalbumin (OVA)-sensitized BALB/c mice. Oral administration with L. reuteri restored the deteriorated profile of enteric flora, and attenuated allergic diarrhoea, mast cell activation, and serum IgE production in allergic mice. The production of signature T helper (Th)1 and 2 cytokines, namely IFN-γ and IL-4, by splenocytes was suppressed by L. reuteri. Concordantly, the intestinal expression of IFN-γ, IL-4, T-bet and GATA3 was down-regulated. However, L. reuteri augmented the expression of IL-10, TGF-β and Foxp3, and the number of IL-10-secreting CD11c+CD103+ mesenteric lymph node (MLN) cells. Furthermore, direct exposure to heat-killed L. reuteri attenuated OVA-induced cell proliferation and IL-2 secretion by MLN cells. These results demonstrate that L. reuteri possesses anti-allergic activities via modulating enteric flora and promoting tolerogenic immune responses, and suggest L. reuteri as a functional probiotic against food allergy. ? 2017 Elsevier LtdFood allergy; Lactobacillus reuteri; Regulatory T cell; Tolerogenic dendritic cell[SDGs]SDG3journal article10.1016/j.jff.2017.01.0342-s2.0-85010377713https://www2.scopus.com/inward/record.uri?eid=2-s2.0-85010377713&doi=10.1016%2fj.jff.2017.01.034&partnerID=40&md5=d8244ad5e35a5b8ee0c9f2f4bbe264ba