2008-07-172024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/684712摘要：水牛是台灣重要的畜牧產業之一，雖然其傳統的役用價值已隨時代變遷而消失，可是其肉用價值、地方特色及遺傳保種價值卻日漸浮現。相較於其他牛種，水牛之繁殖效率仍是低落，然而繁殖效率之高低是影響牧場經營成敗之重要指標，利用生物科技技術有效提升繁殖效能，才能降低成本提升競爭力，進一步振興台灣之水牛產業。在許多哺乳動物研究中已知 Kisspeptin 可刺激下視丘GnRH神經元，進而刺激腦垂腺釋放激性線素 LH，啟動繁殖相關的內分泌系統。本計畫擬應用水牛之 kiss1 基因選殖之大腸桿菌產製人工合成之 Kisspeptin，以注射或皮下埋植方式，刺激下視丘激性線素釋放素（GnRH）的分泌，期以於乏情期間啟動正常動情週期，以縮短胎距。甚或在其他條件配合之下，藉以提前水牛之發身期，將初產年齡提前，提高水牛之繁殖效能。<br> Abstract: The buffalo is one of the important agricultural industries in Taiwan. The traditional value of buffalo in labor works has diminished due to use of mechanics in agriculture sectors, but its value in meat industry, genetic conservation and as a distinguishing characteristic for local development is gradually emerging. Reproduction performance is the major index for the success of a farm management. Using modern biotechnology to increase reproduction efficiency to reduce production cost is the way to promote buffalo industry in Taiwan. It`s been know that Kisspeptin can stimulate GnRH producing neurons in hypothalamus, which can induce pituitary to release LH to activate reproduction related endocrine system in mammal. The main purpose of this research is to use buffalo kiss1 gene to synthesize Kisspeptin peptide. By way of injection or under skin implant, stimulate hypothalamus to secrete GnRH to activate H-P-G axis to resume estrus cycle during non-breeding season. Furthermore, under other suitable conditions, Kisspeptin may also advance puberty time, hence reduce the time of the first pregnancy in buffalo. Both ways can effectively increase reproduction performance in Taiwan buffalo.BuffaloReproductive BiotechPeptideEstrous cycle水牛生殖技術胜&#32957動情周期