2005-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/663884摘要：水稻稻熱病，為水稻栽植國家或地區最重要病害之一。近年來，雖然台灣地區水稻栽培面積日趨減少，但水稻仍為主要之糧食作物，且稻熱病的發生亦時有所聞。本病害防治之重要方法，包括抗病育種及藥劑防治，如何減少藥劑的施用以及因本病原菌快速變異所導致的田間抗藥性菌株之產生或抗病品系的失效等問題，而分析並瞭解田間稻熱病菌品系之分佈及動態變化，將有助於病害發生的預測，田間抗病品系之施用，以及抗病育種之篩選等工作。本計劃之總目標在於，發展本土水稻稻熱病菌菌株品系分子鑑別系統，並藉以調查本土病原品系之分布及特性。本年度目標包括：(1)進行本土田間菌株之分離及培養；(2)進行田間菌株基因體核酸之純化；(3)進行種內分群分子探針之選殖及定序；(4)進行菌株基因體核酸南方雜合分析；(5)進行本土田間菌株種內分群結果之分析。重要工作項目包括：a.繼續利用組織或單孢分離的方法，進行本省水稻第一期稻作田間稻熱病菌株之全面分離、培養及保存；b.進行本土稻熱病菌株基因體核酸之萃取及純化，進行本土田間菌株之基因體核酸之限制酵素切割，電泳，轉漬，並以種內品系分群之MGR分子探針進行南方雜合分析；c.嘗試分離稻熱病菌之其他重覆性序<br> Abstract: Rice blast disease is one of the most devastating diseases of cultivated rice in many developing countries. When the environmental and climate conditions are suitable, the dramatic yield loss will result from the epidemics of the disease. One of the measures to control the disease is to deploy resistance rice cultivar, however, the breakdowns of the host resistance have often been observed due to the frequent genetic variability of the fungus in the field. To understand the genetic variability of this fungus in Taiwan, we propose to use MGR (Magnaporthe grisea repeat) DNA fingerprinting technique to survey the clone lineages of the fungus. The goal of the proposal is hoping to generate the genetic structure of the fungus in Taiwan, use it to monitor the dynamic structure and distribution of the fungus, and also as the information for disease prediction, and screening and deploying of resistant rice cultivars.水稻稻熱病菌病原品系調查RiceMagnaporthe griseaDNA fingerprinting