LU-PING CHOWSu N.-Y.Yu C.-J.BOR-LUEN CHIANGShen H.-D.2020-01-222020-01-22199902646021https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033166498&doi=10.1042%2f0264-6021%3a3410051&partnerID=40&md5=37a9656068ac295349ad10391aec2e96https://scholars.lib.ntu.edu.tw/handle/123456789/452456The mould genus, Penicillium, is known to be a significant source of environmental aero-allergens. One important allergen from Penicillium citrinum, Pen c 2, has been identified by means of two-dimensional immunoblotting using IgE-containing patients' sera. This novel allergen was cloned, sequenced and expressed in Escherichia coli. The cloned cDNA encodes a large 457-amino acid protein precursor containing a 16-amino acid signal peptide, a 120-amino acid propeptide and the 321-amino acid mature protein. Comparison of the Pen c 2 sequence with known protein sequences revealed shared high sequence similarities with two vacuolar serine proteases from Aspergillus niger and Saccharomyces cerevisiae. Asp-46, His-78 and Ser-244 were found to constitute the catalytic triad of the 39-KDa Pen c 2. The DNA coding for Pen c 2 was cloned into vector PQE-30 and expressed in E. coli as a His-tag fusion protein that bound serum IgE from Penicillium-allergic patients on immunoblots. Recombinant Pen c 2 could therefore be used effectively for diagnosis and also potentially for the treatment of mould-derived allergic disorders.[SDGs]SDG3allergen; complementary DNA; hybrid protein; immunoglobulin binding factor; immunoglobulin e; pen c 2 allergen; protein precursor; recombinant protein; serine proteinase; unclassified drug; allergic disease; amino acid sequence; article; aspergillus niger; clone; controlled study; escherichia coli; human; immunoblotting; molecular genetics; nonhuman; nucleotide sequence; penicillium; priority journal; protein expression; saccharomyces cerevisiae; sequence homology; Allergens; Amino Acid Sequence; Antigens, Fungal; Base Sequence; Cloning, Molecular; Epitopes; Humans; Hypersensitivity, Immediate; Immunoglobulin E; Molecular Sequence Data; Penicillium; Recombinant Proteins; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Serine Endopeptidases; Vacuoles; Aspergillus niger; Escherichia coli; Penicillium; Penicillium citrinum; Saccharomyces cerevisiaejournal article10.1042/0264-6021:34100512-s2.0-0033166498