Hsu C.Ho H.-W.Chang C.-F.Wang S.-T.Fang T.-F.Lee M.-H.NAN-WEI SU2020-01-062020-01-06201309639969https://scholars.lib.ntu.edu.tw/handle/123456789/446228Daidzein and genistein have many benefits for human health; however, their applications may be restricted because of their low aqueous solubility. In this work, we generated two water soluble isoflavone derivatives, daidzein 7-O-phosphate and genistein 7-O-phosphate, by incubating Bacillus substilis var. natto BCRC 80517 with daidzein and genistein. These two isoflavone derivatives were characterized by HPLC-ESI-MS/MS, 13C NMR and 31P NMR. Genistein was phosphorylated more rapidly than daidzein. In addition, this bacterial strain could transform glucosidic isoflavones via aglucones into the corresponding 7-O-phosphate conjugates. However, despite the production of agluconic daidzein and genistein, cells could not transform malonyl glucosidic isoflavones into the 7-O-phosphate conjugates. Furthermore, alkaline phosphatase activities from human colon carcinoma (Caco-2) cells were found effective in the dephosphorylation of daidzein 7-O-phosphate and genistein 7-O-phosphate into the corresponding aglyconic isoflavones. ? 2013 Elsevier Ltd.[SDGs]SDG3Bacillus subtilis natto; Biotransformation; Daidzein; Genistein; Isoflavones; Bacteriology; Nuclear magnetic resonance spectroscopy; Phosphatases; Value engineering; Flavonoidsjournal article10.1016/j.foodres.2013.05.0272-s2.0-84879491314https://www2.scopus.com/inward/record.uri?eid=2-s2.0-84879491314&doi=10.1016%2fj.foodres.2013.05.027&partnerID=40&md5=b619b8faf679d37f5a9b36a9925d9e0a