2012-08-012024-05-16https://scholars.lib.ntu.edu.tw/handle/123456789/666483摘要：薊馬體形微小、繁殖力強、寄主範圍廣，不僅直接危害蔬菜、瓜果與花卉，甚至傳播許多病毒性病害。薊馬傳播的番茄斑萎病毒 (tospoviruses) 可以說是為害最多作物種類的病毒之一。近年來，台灣南部的洋香瓜栽培飽受植物病毒病害的威脅，引起農政單位的重視，其中一項重要的病原即是南黃薊馬 (Thrips palmi) 傳播的西瓜銀斑病毒 (Watermelon silver mottle virus, WSMoV)。國外與 tospovirus相關的研究多以番茄斑萎病毒 (Tomato spotted wilt virus) 及其重要病媒—西方花薊馬 (Frankliniella occidentialis) 作為研究主題；我們希望利用此計畫深入研究另一病媒—病毒系統 (南黃薊馬—西瓜銀斑病毒)，以作為日後比較研究之用。這個研究計畫的主要目的利用生態學實驗與新穎的新世代基因解序分析 (Next Generation Sequencing)，來研究南黃薊馬與 WSMoV 之間的交互作用。我們將探討 WSMoV 對南黃薊馬生殖力、發育速度、成蟲壽命等生活史特徵的影響，以及感染 WSMoV 對南黃薊馬寄主植物取食與產卵偏好性的影響。我們將以新世代基因解序分析來探究南黃薊馬在分子層次上對 WSMoV 感染的反應，並利用昆蟲組織學的實驗，確認 WSMoV 感染南黃薊馬的組織與薊馬體內傳播途徑。最後，利用重組病毒蛋白與薊馬中腸細胞的附著，鑑別與 WSMoV 的病媒傳播有關的病毒蛋白；並以人工餵食蛋白質競爭試驗，探討重組病毒蛋白抑制病毒傳播的可能性。以發展潛力來看，重組病毒蛋白也可以與小分子毒性物質結合，發展出只殺死特定昆蟲的專一性生物殺蟲劑。藉由跨領域的合作，希望可以促進農業科技創新性之研究發展。<br> Abstract: Thrips have small body size, high reproductive rate and wide host range. They not only damage vegetables, fruits and flowers, but also transmit many viral diseases to crops. Thrips-borne tospoviruses are one of the most destructive plant viruses. Recently, viral diseases threaten the cultivation of muskmelons in southern Taiwan causing the attention of agricultural authorities. One of the important pathogens is thrips-transmitted Watermelon silver mottle virus (WSMoV). Tomato spotted wilt virus and its key vector, Frankliniella occidentialis, is the well-studied system in western countries. We propose studying another thrips-virus system (Thrips palmi-WSMoV) for future comparative works. The main objective of this research proposal is to study the interactions between Thrips palmi and WSMoV using ecological experiments and Next Generation Sequencing (NGS). We will study the influence of WSMoV infection on the life history of Thrips palmi, such as reproductive rate, developmental rate and adult longevity. We also plan to compare the host preference of healthy and WSMoV-infected thrips in both feeding and oviposition aspects. We will use NGS to study the molecular response of Thrips palmi to WSMoV infection and histological experiments to identify the virus-infected tissues and virus translocation route in thrips. Finally, in vivo binding assay of recombinant viral proteins in thrips midgut will identify viral protein(s) related to the vector transmission of WSMoV. Further, acquisition competition assay will exam whether recombinant viral proteins inhibit the transmission of the virus by Thrips palmi. A small toxic molecule can attach to the recombinant viral proteins to develop a species-specific pesticide. Through interdisciplinary collaboration, we hope to promote the innovation and development of agricultural science and technology.生活史新世代基因解序分析番茄斑萎病毒屬南黃薊馬西瓜銀斑病毒life historynext generation sequencingTospovirusThrips palmiWatermelon silver mottle virus