DNA binding activity of nuclear factor of activated T cells in mononuclear cells from renal transplant patients with and without BK virus viruria

Yin W.-Y.Lai N.-S.Lee M.-C.CHIA-LI YUHuang S.-L.Lu M.-C.2021-01-272021-01-2720131016-3190https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878152925&doi=10.1016%2fj.tcmj.2013.04.001&partnerID=40&md5=11c289cfeddb4d9ff6d8163dfd33b4f1https://scholars.lib.ntu.edu.tw/handle/123456789/542179Objectives: Renal transplant patients receive calcineurin inhibitors to suppress the calcineurin-nuclear factor of activated T cells (NFAT) pathway. The DNA binding activity of NFAT and its relationship to the reactivation of BK virus (BKV) has not been evaluated in renal transplant patients. Patients and Methods: The DNA binding activity of NFAT cytoplasmic 1 (NFATc1) was measured by enzyme-linked immunosorbent assay in peripheral blood mononuclear cells from 26 renal transplant patients and 26 healthy controls. At the same time, their urinary BKV viral load was measured by real-time polymerase chain reaction. Results: The activity of NFATc1 was lower in renal transplant patients without BKV viruria [BKV (-)] than in healthy controls, while it trended to be higher in renal transplant patients with BKV viruria [BKV (+)] than in BKV (-) patients. The tacrolimus blood levels did not differ between BKV (+) and BKV (-) renal transplant patients or correlate with NFATc1 activity. Conclusion: NFATc1 DNA binding activity was lower in renal transplant patients without BKV viruria than in those who were BKV (+). However, there was no relationship between tacrolimus blood levels and NFATc1 activity in renal transplant patients. ? 2013, Buddhist Compassion Relief Tzu Chi Foundation.BK virus; Calcineurin nuclear factor of activated T cells; Renal transplantation; Tacrolimus[SDGs]SDG3cyclosporin; mycophenolic acid; mycophenolic acid 2 morpholinoethyl ester; prednisolone; rapamycin; tacrolimus; transcription factor NFAT; transcription factor NFATc1; unclassified drug; adult; age distribution; article; BK virus; bk virus infection; clinical article; controlled study; correlation analysis; DNA binding; enzyme linked immunosorbent assay; female; gene dosage; graft recipient; human; kidney transplantation; male; peripheral blood mononuclear cell; real time polymerase chain reaction; sex difference; virus load; virus reactivationDNA binding activity of nuclear factor of activated T cells in mononuclear cells from renal transplant patients with and without BK virus viruriajournal article10.1016/j.tcmj.2013.04.0012-s2.0-84878152925