Jen Tien C.Chiang B.H.2019-07-112019-07-11199913595113https://scholars.lib.ntu.edu.tw/handle/123456789/413333Colloid particles, produced by mixing H2SO4 and ZrOCl2 were treated with 3-aminopropyltriethoxysilane (3-APTES) and glutaraldehyde and deposited on a ceramic tube to form a dynamic membrane. £\-Amylase was immobilized onto the membrane by means of covalent coupling to the glutaraldehyde pretreated colloids. The process, using 0.5% glutaraldehyde, produced an active dynamic membrane with an activity of 59.8 U/g wet gel. The specific activity of £\- amylase on the membrane was 21.85 U/mg protein at its optimum pH 5.5 and 41¢XC, compared with the highest specific activity, 1.91 U/mg protein, of the free enzyme at the optimum pH 6.6 and 63¢XC. The membrane should be used at a temperature below 40¢XC to maintain enzyme activity.£\-AmylaseCeramicDynamic membraneImmobilization enzymeZirconiumjournal article10.1016/S0032-9592(99)00081-32-s2.0-0033229723https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033229723&doi=10.1016%2fS0032-9592%2899%2900081-3&partnerID=40&md5=ee5ecda0047d47fa36d98b79be63609c