Wu, Pei ChunPei ChunWuGuo, Lun ZhangLun ZhangGuoYu, ShanShanYuZeng, NingNingZengLiu, Yu ChengYu ChengLiuYu, JiaJiaYuZhang, ZhimingZhimingZhangLu, KeKeLuSun, LiangyuLiangyuSunWang, ChunfeiChunfeiWangChang, Yu HanYu HanChangLu, Yin LinYin LinLuShen, Yu FangYu FangShenTai, ShengShengTaiYUEH-HSUN CHUANGAnnie Ho, Ja AnJa AnAnnie HoKAI-WEN HUANGYAO-MING WULiu, Tzu MingTzu MingLiu2022-04-142022-04-142022-04-012156-7085https://scholars.lib.ntu.edu.tw/handle/123456789/604244Using in vivo multiphoton fluorescent dosimetry, we demonstrate that the clearance dynamics of Indocyanine Green (ICG) in the blood can quickly reveal liver function reserve. In normal rats, the ICG retention rate was below 10% at the 15-minute post-administration; While in the rat with severe hepatocellular carcinoma (HCC), the 15-minute retention rate is over 40% due to poor liver metabolism. With a 785 nm CW laser, the fluorescence dosimeter can evaluate the liver function reserve at a 1/10 clinical dosage of ICG without any blood sampling. In the future, this low-dosage ICG 15-minute retention dosimetry can be applied for the preoperative assessment of hepatectomy or timely perioperative examination.journal article10.1364/BOE.4467492-s2.0-85126987923https://api.elsevier.com/content/abstract/scopus_id/85126987923