Hsu, T.-H.T.-H.HsuKao, Y.-L.Y.-L.KaoLin, W.-L.W.-L.LinXiao, J.-L.J.-L.XiaoKuo, P.-L.P.-L.KuoWu, C.-W.C.-W.WuWEI-YU LIAOPO-LING KUO2020-07-212020-07-2120121757-969417579694https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863027093&doi=10.1039%2fc2ib00112h&partnerID=40&md5=621d41d3c6af2015b96a624035c699abhttps://scholars.lib.ntu.edu.tw/handle/123456789/510305We employ a microfluidic chip with three culture chambers to investigate the interactions among lung cancer cells, macrophages and myofibroblasts. By mixing the conditioned media of macrophages and myofibroblasts in this chip, we confirm that these two stromal cells have synergistic effects in accelerating the migration of cancer cells. However, as the myofibroblasts are pretreated with the conditioned medium of macrophages, the myofibroblasts' ability to enhance the migration of cancer cells is lowered. The tumour necrosis factor-α produced by macrophages reduces the expression of α-smooth muscle actin and the secretion of transforming growth factor-β1 in myofibroblasts. Once the tumour necrosis factor-α in the macrophage conditioned medium is neutralized, the macrophage medium-pretreated myofibroblasts can still accelerate the migration of cancer cells. ? 2012 The Royal Society of Chemistry.[SDGs]SDG3alpha smooth muscle actin; transforming growth factor beta1; tumor necrosis factor alpha; article; biochip; cancer cell; cancer cell culture; cell migration; coculture; controlled study; human; human cell; macrophage; microchip analysis; microfluidics; myofibroblast; priority journal; protein expression; protein secretion; stroma cell; Adenocarcinoma; Cell Communication; Cell Line, Tumor; Cell Movement; Coculture Techniques; Culture Media, Conditioned; Humans; Lung Neoplasms; Macrophages; Microfluidics; Microscopy, Confocal; Myofibroblasts; Transforming Growth Factor beta; Tumor Necrosis Factor-alphajournal article10.1039/c2ib00112h221794252-s2.0-84863027093