廖中明Liao, Chung-Min臺灣大學:生物環境系統工程學研究所趙聖峰Jau, Sheng-FengSheng-FengJau2010-05-052018-06-292010-05-052018-06-292008U0001-2706200817140800http://ntur.lib.ntu.edu.tw//handle/246246/181131本論文主要研究目的在於評估淡水蜆(Corbicula fluminea)暴露於砷之蜆殼律動毒性反應及生物動力。本研究完成實驗室14天之暴露試驗，並以一階單區塊模式擬合暴露試驗資料推估生物濃縮因子(bioconcentration factor, BCF)及排除速率常數(depuration rate constant, k2)等生物動力參數。本研究以關鍵體內濃度(critical body residue, CBR)模式、關鍵濃度曲線面積(critical area under the curve, CAUC)模式及損害評估模式(damage assessment model, DAM)等三種急性毒模式模擬7天急性砷毒生物試驗所求得之外部半致死濃度(external median lethal concentration, LC50)與時間關係的資料。本研究亦發展一套以感應線圈為基礎之蜆殼開闔監測技術特性化淡水蜆暴露於砷之每日蜆殼律動，並以三參數Hill模式推估半數蜆殼反應之效應濃度(median effect concentration, EC50)與時間之關係。結果顯示淡水蜆暴露於砷之BCF, k2及96小時LC50值分別為4.38 mL g-1、0.39 d-1 及 20.74 mg L-1。本研究採四參數正弦函數擬合無砷汙染下蜆殼每日開殼律動情形，同時結合Hill反應函數建構出淡水蜆暴露於砷之蜆殼每日律動方程式。結果指出一天中蜆殼開殼機率之最佳描述式可表示為 。經由推估所得砷暴露下時變之EC50值於整合時間10、15、30、60、120及300分鐘下分別為4.65、3.48、1.38、0.60、0.38及0.35 mg L-1。模擬結果顯示在蜆殼開殼時間為3:00 – 8:00時，在不同砷暴露濃度0.3 ~ 1.0 mg L-1下預測之淡水蜆每日蜆殼開殼變化與實驗結果非常穩合，其均方根誤差為4 – 10 %。此外，本研究結合DAM模式及Hill模式建構以內部效應濃度為基礎之時變致死率模式，進而推估出淡水蜆體內砷含量與致死率之關係。本研究指出淡水蜆暴露於砷將導致行為改變及死亡情形，於日後可提供淡水蜆養殖場一水域含砷之品質標準。The purpose of this thesis is to assess the biokinetics and toxicological response of valve daily rhythms in the freshwater clam Corbicula fluminea following exposure to waterborne arsenic. We carried out a laboratory 14-day exposure experiment to obtain biokinetic parameters of bioconcentration factor (BCF) and depuration rate constant (k2). A one-compartment first-order model was used to fit the exposure data to estimate BCF and k2 values. The three acute toxicity models, critical body residue (CBR) model, critical area under the curve (CAUC) model and damage assessment model (DAM) were verified with time-dependent external median lethal concentration (LC50) data obtained from a 7-day acute arsenic toxicity bioassay. We develop an inductance-based valvometry technique to characterize the valve daily rhythms in C. fluminea in response to arsenic, and use a three-parameter Hill model to estimate intergration time-specific median effect concentration (EC50) values. The resulting values of BCF, k2 and 96-h LC50 of C. fluminea exposure to arsenic were 4.38 mL g-1, 0.39 d-1 and 20.74 mg L-1, respectively. We fitted valve opening daily rhythm in the absence of arsenic as the form of by 4-parameter sine function, linking Hill response function to predict valve daily rhythm dynamics in response to arsenic. The time-varying EC50s are estimated to be 4.65, 3.48, 1.38, 0.60, 0.38 and 0.35 mg L-1, respectively, at integration times of 10, 15, 30, 60, 120 and 300 min. The simulation results show that in the valve opening hours from 03:00 – 08:00 h the predicted daily rhythm changes in valve opening in response to different arsenic exposure concentrations ranging from 0.3 to 10 mg L-1 are notably agreed well with the observations justified by the root mean squared errors (RMSE) ranging from 4 – 10%. Moreover, we also kinetically linked a DAM model and a Hill sigmoid model to reconstruct an internal effect concentration based time-mortality model assessing the effect of soft tissue arsenic burden on the C. fluminea mortality. Our study indicates that C. fluminea exposed to arsenic will cause behavioral changes and mortality, suggesting that our results can provide the knowledge to establish the arsenic water quality criteria to clam farm.中文摘要 I文摘要 III錄 V目錄 VII目錄 VIII號說明 X一章 前言 1二章 研究背景與目的 2.1 研究背景 2 2.2 研究目的 3三章 文獻回顧 4.1 淡水蜆生態毒理 4 3.1.1 淡水蜆(Corbicula fluminea) 4 3.1.2 淡水蜆對重金屬之生物累積 7.1.3 重金屬對蜆殼律動行為之改變 10.1.4 砷對淡水蜆之影響 14.2 生態毒理模式 16.2.1 生物累積模式 16.2.2 急性毒模式 18.2.3 劑量反應模式 23.2.4 急毒生物實驗 24四章 材料與方法 26.1 淡水蜆之採樣及馴養 28.2 暴露實驗 29.3 急毒生物試驗 30 4.4 蜆殼每日律動試驗 31.4.1 實驗裝置與原理 31.4.2 蜆殼行為生物試驗 36 4.5 化學分析與資料處理 37.5.1 總砷含量與離子分析 37.5.2 淡水蜆體內砷含量分析 38.5.3 淡水蜆殼律動資料處理 39 4.6 數學模式之建構與發展 41.6.1 生物累積模式之應用 41.6.2 內部效應濃度為基礎之時變致死率模式 43.6.3 蜆殼律動模式 45五章 結果與討論 48.1 淡水蜆對砷之生物濃縮 48.2 急毒效應 54.3 急性毒模式應用 60.4 內部半致死與時變致死率 64.5 淡水蜆每日律動特性 69.6 蜆殼行為效應 77.7 水域環境之重金屬監測 89.8 溫度對蜆殼行為及重金屬累積之影響 93六章 結論 97七章 未來研究建議 99考文獻 101application/pdf4257972 bytesapplication/pdfen-US淡水蜆砷生物累積急性毒劑量反應貝殼行為活動生物監測Freshwater clamCorbicula flumineaArsenicBioaccumulationAcute toxicityDose-responseValve movementBiomonitorthesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/181131/1/ntu-97-R95622016-1.pdf