2006-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/660501摘要：ABC 蛋白廣泛的存在於各種有機體中，是基因成員最多的基因家族之一，大部分ABC 蛋白具有穿 膜序列(TMD)的膜蛋白，由於這些膜蛋白都有保守性的ATP-binding cassette，因此稱為ATP-binding cassette transporter(ABC transporter)，ABC 轉運蛋白與細胞膜大多數基質的進出息息相關。植物中， 阿拉伯芥共有131 個基因ABC 轉運蛋白，這些轉運蛋白在依照基因親源關係及功能區域結構的差 異，分成許多次基因家族，功能較為清楚的是MDR、PDR，和WBC 這個次基因家族，其中MDR 次基因家族和多重除草劑抗性及Auxin 運輸有關。PDR 基因家族和二次代謝物分泌、植物防禦、 及逆境反應有關。WBC 基因則和細胞蠟質分泌、雙子葉植物Ｋanamycin 抗性有關。植物基因轉殖 過程中，常用的篩選用標誌基因(包括抗生素和除草劑抗性基因)，大都來自微生物，因而造成大眾 <br> Abstract: ABC proteins are one of the largest gene families and are ubiquitiously found in all living organisms. Most ABC proteins are transporters, which share a highly conserved ATP-binding cassette and are consisting of one or double set of two basic structural elements, a transmembrane domain and an ATP-binding cassette. The ABC transporters are involved in the import or the export of a wide variety of substances. In Arabidopsis, 131 ABC transporters have been identified and have been classified into several subfamilies corresponding to phylogenetic pathways and structural features. Functions of ABC transporters of several subfamilies have been characterized, such as MDR, PDR, and WBC subfamily. Most of genes belong to the MDR subfamily are involved in multiherbicide tolerance and auxin transport. Genes in the PDR subfamily are related to plant defense, secondary metabolite secretion, and stress responses. The genes of WBA subfamily play a role in the wax secretion and kanamycin resistance of Arabidopsis. In the past twenty years, selection markers have been extensively used in plant transformation. However, the GMO controversy continues due to the concern of the horizontal gene transfer from the microbial genes and the creation of superweeds. To prevent the public concern from GMO issue, using of plant’s native gene as a selection marker maybe a promising route. In this study,rice ABC transporter genes belongs to MDA, PDR, and WBC subfamilies were identified via in silico analysis according to the conserved domains from Arabidopsis. To functional analysis of the rice ABC transporters, two strategies will be pursued. The first way is analyzing the T-DNA activation tagging/Knock out rice. The second approach is the overexpression of rice ABC transporters. We are aimed to screen the antibiotics (hygromycin) or herbicide (L-phosphinothricin) resistant genes that could be used as a non-microbial origin selection marker in the future. Emphasis is also being placed on the screening of stress resistant genes that could be used to improve stress tolerance of crops.ABC 轉運蛋白功能分析篩選標誌轉殖水稻ABC transporterFunctional analysisSelection markerTransgenic rice水稻ABC(ATP-binding cassette)轉運蛋白超家族中逆境、抗生素、及除草劑抗性基因之篩選與應用