Yang, Wen-ChunWen-ChunYangHsu, Tsui-ChunTsui-ChunHsuKUAN-CHEN CHENGJE-RUEI LIU2019-07-112019-07-112017-04-2414752859https://www.scopus.com/record/display.uri?eid=2-s2.0-85018564546&origin=recordpagehttps://scholars.lib.ntu.edu.tw/handle/123456789/413419Article number: 69Background: Mycotoxins are secondary metabolites produced by filamentous fungi that can contaminate agricultural crops in the field as well as during harvest, transportation, processing, or storage. Zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, produced by Fusarium species, has been shown to be associated with reproductive disorders in farm animals and to a lesser extent in hyperoestrogenic syndromes in humans. Thus, the decontamination of ZEN in foods and feeds is an important issue. Results: In this study, the gene encoding ZHD101, a ZEN-degrading enzyme produced by Clonostachys rosea IFO 7063, was cloned into an Escherichia coli-Lactobacillus shuttle vector, pNZ3004, and the resultant plasmid pNZ-zhd101 was then introduced via electroporation into Lactobacillus reuteri Pg4, a probiotic strain isolated from the gastrointestinal tract of broilers. The transformed strain L. reuteri pNZ-zhd101 acquired the capacity to degrade ZEN. In addition, the production of recombinant ZHD101 did not affect cell growth, acid and bile salt tolerance, and had only a minor effect on the adhesion ability of L. reuteri pNZ-zhd101. Conclusions: To the best of our knowledge, this is the first report of successful expression of a ZEN-degrading enzyme by intestinal lactobacilli. ? 2017 The Author(s).Lactobacillus reuteriLactonohydrolaseProbioticsZearalenone[SDGs]SDG2[SDGs]SDG11journal article10.1186/s12934-017-0687-82-s2.0-85018564546