Hsieh, H.-H.H.-H.HsiehHo, L.-C.L.-C.HoHUAN-TSUNG CHANG2018-09-102018-09-102016http://www.scopus.com/inward/record.url?eid=2-s2.0-84986230542&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/397753We have developed a simple route for the preparation of aminophenylboronic acid polymer nanoparticles (APB PNs) from 3-aminophenylboronic acid and formaldehyde under alkaline conditions according to an extended St?ber method. Insulin and R6G have been selected to prepare functional insulin-APB PNs and R6G-APB PNs, respectively. During the formation of APB PNs, the representative molecules are embedded inside the APB PNs. Through specific binding of glucose with boronic acid moieties on the R6G-APB PNs and insulin-APB PNs, glucose induces expansion of the APB PNs, leading to release of R6G and insulin molecules, respectively. As a result of release of R6G molecules, the fluorescence intensity of R6G-APB PN solution increases, allowing quantitation of glucose in PBS solutions (10 mM, pH 7.4) with a linear range over 0–10 mM. Release of insulin from insulin-APB PNs is significant and rapid when the glucose concentration is higher than 7 mM. Having advantages of low cost, simple preparation, biocompatibility, and continuous response to glucose, the insulin-APB PNs hold great potential as an alternative for treating diabetic patients. [Figure not available: see fulltext.] ? 2016, Springer-Verlag Berlin Heidelberg.Boronic acid; Glucose detection; Insulin release; Polymeric nanoparticles[SDGs]SDG3Alkalinity; Biocompatibility; Glucose; Molecules; Nanoparticles; Polymers; 3-Aminophenylboronic acid; Aminophenylboronic acid; Boronic acid; Fluorescence intensities; Glucose concentration; Glucose detection; Insulin release; Polymeric nanoparticles; Insulin; 3-aminobenzeneboronic acid; boronic acid derivative; fluorescent dye; insulin; nanoparticle; rhodamine; rhodamine 6G; adult; analysis; chemistry; female; glucose blood level; human; procedures; spectrofluorometry; ultrastructure; Adult; Blood Glucose; Boronic Acids; Female; Fluorescent Dyes; Humans; Insulin; Nanoparticles; Rhodamines; Spectrometry, Fluorescencejournal article10.1007/s00216-016-9842-z2-s2.0-84982105890WOS:000382883300004