2023-08-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/665365Bone is a highly active tissue that undergoes continuous remodeling throughout devel-opment and adulthood to maintain structural integrity and repair upon injury. Osteo-genesis is the process by which progenitor cells differentiate into the osteoblastic line-age and is central to bone growth and homeostasis. While many chemical and physical osteogenic stimuli have been characterized for their functions in signaling and pheno-typic regulation, their impact on metabolic or organelle reprogramming remains unclear. Mitochondria function is closely related with aging, and mediates age-related bone loss through reducing osteoblast population and mineralized matrix formation. In this pro-posal, we will investigate the role of mitochondria, the energy and signaling hub, in osteogenesis, and explore the molecular underpinning. We will establish live mitochon-drial imaging platforms in mesenchymal stem cells (MSCs) and quantify the effects chemical and physical osteoinduction cues on mitochondrial structure and function. We will also investigate the underlying molecular mechanisms by screening with a panel of small molecule inhibitors. Mechanistic understanding of mitochondrial regulation in osteogenesis will shed light in bone homeostasis and provide therapeutic targets in os-teoporosis and fracture treatments.再生醫學;成骨作用;粒線體;生物材料;奈米科技;Regenerative medicine; osteogenesis; mitochondria; biomaterials; nanotechnology