2016-08-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/649044摘要：國內104-105流感流行季出現較往年更多的流感重症病例，依據國內監測資料顯示104年7月1日至105年4月20日，累計1,960例流感併發重症病例，其中推估352例與流感相關死亡。整體疫情較前3年嚴重，為釐清此波流感疫情原因，以及各項因應作為之妥適性，以利強化未來流感防治政策調整之實證依據。本計畫目的為瞭解我國6個月至12歲孩童之流感抗體相關血清流行病學資料，並與其接種史作關連性分析，以探討104-105年流感季疫情的可能原因，並分析疫苗之效益。所得資料作為未來預測流行病毒、疫情狀況及疫苗接種等政策調整之參考依據。本實驗室欲收集104年8月至105年2月，且年紀介於6個月至12歲的幼童，區分為學齡前幼兒（6個月至未滿6歲）及學齡兒童（6歲至未滿12歲）之血清檢體為材料，共約100支，進行抗體檢測(利用HAI assay，針對H1N1, H3N2, B Vitoria, B Yamagata，此四型流感病毒)，以瞭解研究對象對流感的免疫力情形，之後配合相關人口學資料並對照其疫苗接種紀錄，或搜查病歷是否有流感症狀就醫紀錄，主要想釐清以下問題：(1) 流感季前兒童血清抗體效價是否可評估當季流感流行幅度或有無關聯性?；(2) 若有施打疫苗的紀錄，其流感疫苗是否提供足夠的保護力?藉由分析幼童的抗體免疫反應，得到實證資料並提出各項具體政策建議。<br> Abstract: keywords：influenza virus, antibody responses, flu vaccineAccording to Taiwan CDC, there were more severe influenza cases than before in 2015-2016 influenza season in Taiwan. Since the beginning of this influenza season on July 1, 2015, as of April 20, 2016, a total of 1,960 severe influenza cases, including 352 deaths, have been confirmed. The overall epidemic situation is more serious than past 3 years. In order to strengthen the control of influenza in Taiwan, we need to gather evidence to understand what are the reasons behind this unexpected large-scale influenza epidemic and could we predict this in the future?The aim of this project is to investigate the influenza seroepidemiology of children 6 months to 12 years age in Taiwan. We could also understand the antibody titers following vaccination based on the vaccination history. We previously showed that lack of antibody to certain influenza subtype in children is an indication that this subtype would cause a substantial outbreak in the coming season. We are interested to see if the preseason sera from children lack antibody to all the 4 circulating influenza strains. We will include 100 serums from children aged between 6 months and 12 years of age in this study. They were collected between August, 2015 and February, 2016 and will be divided into pre-school children (under the age of 6 months to 6 years old) and school-age children (6 years old to under 12 years). The HAI titers against 4 strains (H1N1, H3N2, B Vitoria, B Yamagata) will be determined. This study will shed light on the immunity status against influenza virus before the last influenza season. Some of the children had been vaccinated with seasonal influenza vaccine in 2015-2016 season. We can also understand if this vaccine could induce good antibody response in children. We anticipate that the following questions could be addressed: (1) Whether the pre-season children's serum antibody titers of influenza virus could predict the magnitude of seasonal influenza epidemic? (2) Whether the flu vaccines provide enough immunity to protect children?流感流感疫苗血清抗體