LIN-CHI CHEND. K. YangC. F. ChouL. C. Chen2019-08-142019-08-142018https://scholars.lib.ntu.edu.tw/handle/123456789/416486In this work, we investigated aptamer selection against alpha-methylacyl-CoA racemase (AMACR) with three DNA libraries bearing different primers. Because of increased selection diversity, aptamers with varied structural properties, such as pre-folded, induced-folding, high and low primer-dependent conformations, were discovered. From the selection results, a dimeric aptamer was constructed and capable of detecting over-expression of AMACR in prostate cancer cell lines. In summary, this study demonstrates a library-based approach to obtain aptamers with different binding properties and provides distinct aptamers for flexible design of AMACR detection. ? The Royal Society of Chemistry 2018.[SDGs]SDG3Binding energy; Cell culture; Diseases; Aptamers; Binding properties; DNA library; Flexible designs; Methylacyl-CoA racemase; Over-expression; Prostate cancer cells; Selection diversity; Librariesjournal article10.1039/c8ra01808aWOS:000433391600035