Yu, Y.-L.Y.-L.YuChen, I.-H.I.-H.ChenShen, K.-Y.K.-Y.ShenHuang, R.-Y.R.-Y.HuangWang, W.-R.W.-R.WangChou, C.-J.C.-J.ChouChang, T.-T.T.-T.ChangChu, C.-L.C.-L.Chu2021-02-032021-02-03200900142980https://scholars.lib.ntu.edu.tw/handle/123456789/546381Dendritic cells (DC) play a central role in the initiation and regulation of immune responses. Increasing evidence has indicated that manipulation of DC can serve as a therapeutic mechanism for immunomodulation. In this study we tested some unique compounds isolated from Antrodia cinnamomea, a medicinal fungus in Taiwan, on mouse bone marrow-derived DC activation. A triterpenoid methyl antcinate K (me-AntK) promoted DC maturation by enhancing the expression of MHC class II, CD86, and reducing the endocytosis. TNF-alpha, MCP-1, and MIP-1beta were secreted by DC after me-AntK treatment, indicating augmentation of innate immunity by me-AntK. Interestingly, the me-AntK-activated DC induced Ag-specific T-cell proliferation and facilitated Th2 differentiation. Examining signaling responses, we found that me-AntK treatment uniquely activated JNK and ERK in DC. Our results demonstrate that me-AntK is the first natural triterpenoid to promote the ability of DC to prime Th2 responses. This suggests that me-AntK can potentially be applied to enhance immune responses and modulate DC function in immunotherapy.animationURSOLIC ACID; NATURAL-PRODUCTS; IMMUNE-RESPONSE; IFN-GAMMA; T-CELLS; IN-VIVO; SP-NOV; CAMPHORATA; MATURATION; MACROPHAGESjournal article10.1002/eji.200839039197018882-s2.0-70349232883WOS:000269786000021http://www.scopus.com/inward/record.url?eid=2-s2.0-70349232883&partnerID=MN8TOARS19179452