Merit of integrating in situ transcriptomics and anatomical information for cell annotation and lineage construction in single-cell analyses of Populus

Chen, Ying-LanYing-LanChenHsieh, Jo-Wei AllisonJo-Wei AllisonHsiehKuo, Shang-CheShang-CheKuoKao, Chung-TingChung-TingKaoTung, Chia-ChunChia-ChunTungYu, Jhong-HeJhong-HeYuChang, Tien-HsienTien-HsienChangKu, ChuanChuanKuXie, JianboJianboXieZhang, DeqiangDeqiangZhangLi, QuanziQuanziLiYING-CHUNG LIN2024-04-222024-04-222024-04-0314747596https://scholars.lib.ntu.edu.tw/handle/123456789/641990Cell type annotation and lineage construction are two of the most critical tasks conducted in the analyses of single-cell RNA sequencing (scRNA-seq). Four recent scRNA-seq studies of differentiating xylem propose four models on differentiating xylem development in Populus. The differences are mostly caused by the use of different strategies for cell type annotation and subsequent lineage interpretation. Here, we emphasize the necessity of using in situ transcriptomes and anatomical information to construct the most plausible xylem development model.en[SDGs]SDG2[SDGs]SDG13Merit of integrating in situ transcriptomics and anatomical information for cell annotation and lineage construction in single-cell analyses of Populusjournal article10.1186/s13059-024-03227-5385708512-s2.0-85189550876https://api.elsevier.com/content/abstract/scopus_id/85189550876