YING-SHENG LINMousavi, Seyed AbolghasemSeyed AbolghasemMousaviChen, Hung-ChiHung-ChiChen2023-09-082023-09-082022-0817486815https://scholars.lib.ntu.edu.tw/handle/123456789/635281Introduction: Patients with head and neck cancer may suffer from xerostomia after repetitive surgery or radiation therapy. Free colon tissue transfer was used to restore the lubrication of the oral cavity, and a bacteriological study in mice was conducted to evaluate how the intestine transfer from the intra-abdominal to extra-abdominal locations may affect bacterial colonization. Patients and methods: Twelve patients received free colon transfer for the reconstruction of their intraoral mucosal defect following trismus release. Thirty-five patients received ileocolon flap transfer to fill the pharyngeal defects. In an animal study with mice, a pedicled ileum segment was transferred to the subcutaneous space of the abdominal wall. The transferred ileum segments were biopsied for bacterial genome analysis after 3 months. Results: All but one transferred flap survived. Colon secretion was suitable for oral lubrication without the unpleasant smell 1 month postoperatively. In an animal study, genome analysis showed that the same bacterial species still existed in the transferred intestinal tissue. Conclusions: No unpleasant smell or infections were noted in this clinical series despite the animal study showing that the bacterial spectrum did not change in the transferred intestine. Therefore, free colon tissue transfer could safely provide lubrication in patients with xerostomia.enFree colon flapHead neck cancerMicrosurgeryReconstructionXerostomia[SDGs]SDG3journal article10.1016/j.bjps.2022.04.019355992162-s2.0-85130425879