Huang, C.-Y.C.-Y.HuangSu, J.-H.J.-H.SuLiaw, C.-C.C.-C.LiawSung, P.-J.P.-J.SungChiang, P.-L.P.-L.ChiangHwang, T.-L.T.-L.HwangCHANG-FENG DAISheu, J.-H.J.-H.Sheu2018-09-102018-09-102017http://www.scopus.com/inward/record.url?eid=2-s2.0-85029909047&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/400486A continuing chemical investigation of the ethyl acetate (EtOAc) extract of a reef soft coral Sinularia brassica, which was cultured in a tank, afforded four new steroids with methyl ester groups, sinubrasones A–D (1–4) for the first time. In particular, 1 possesses a β-D-xylopyranose. The structures of the new compounds were elucidated on the basis of spectroscopic analyses. The cytotoxicities of compounds 1–4 against the proliferation of a limited panel of cancer cell lines were assayed. The anti-inflammatory activities of these new compounds 1–4 were also evaluated by measuring their ability to suppress superoxide anion generation and elastase release in N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLP/CB)-induced human neutrophils. Compounds 2 and 3 were shown to exhibit significant cytotoxicity, and compounds 3 and 4 were also found to display attracting anti-inflammatory activities. ? 2017 by the authors.Anti-inflammatory activity; Cytotoxic activity; Sinularia brassica; Soft coral; Steroid[SDGs]SDG3antiinflammatory agent; beta dextro xylopyranose; cytochalasin B; elastase; ether derivative; formylmethionylleucylphenylalanine; idelalisib; plant extract; sinubrasone A; sinubrasone B; sinubrasone C; sinubrasone D; Sinularia brassica extract; steroid; unclassified drug; acetic acid derivative; acetic acid ethyl ester; nonsteroid antiinflammatory agent; pancreatic elastase; steroid; superoxide; xylopyranose; xylose; Anthozoa; antiinflammatory activity; Article; cell proliferation; controlled study; correlation spectroscopy; cytotoxicity; enzyme release; heteronuclear multiple bond correlation; human; human cell; IC50; neutrophil count; Sinularia brassica; spectroscopy; analogs and derivatives; animal; chemistry; drug effect; isolation and purification; metabolism; neutrophil; Acetates; Animals; Anthozoa; Anti-Inflammatory Agents, Non-Steroidal; Humans; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Pancreatic Elastase; Steroids; Superoxides; Xylosejournal article10.3390/md150902802-s2.0-85029909047WOS:000411559800019