2005-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/657775摘要：樟芝 (Antrodia cinnamomea)，是臺灣特有之珍貴藥用菌種，目前為止，已有一些研究論文報導指出，樟芝具有抗癌或調節免疫等多方面之活性。此外，由於它僅生長於保育類牛樟的中空心材內壁上，目前尚無法以人工大量栽培子實體，因而更彰顯出樟芝子實體生產的重要性。目前，本實驗室已建構樟芝菌絲體與子實體表現序列標籤(Expressed Sequence Tags, ESTs) 之基因庫及其表現差異之蛋白質體學分析，經由基因鑑定分析結果顯示，已於子實體中獲得一些與脂質、胺基酸和二次代謝生成等未在菌絲體中表現之蛋白質。因此，本研究擬將進一步利用系統生物學之研究策略，研發樟芝之出菇技術，從已建構的蛋白質圖譜中，利用牛樟木材之代謝產物來篩選與樟芝出菇相關之基因，並進行基因調控分析，探討樟芝與牛樟代謝產物之間的相互作用，進而探討菌絲體轉變成子實體之過程，期能克服樟芝出菇之瓶頸。期望在代謝體學、基因晶片、轉殖技術及生物資訊學等配合下，尋找與出菇表現有關之蛋白質及其啟動子，以達成調控樟芝出菇的目標，進而減少保育類牛樟的盜伐，以及藥物開發之應用。<br> Abstract: Chang-Zhi (Antrodia cinnamomea) is an endemic fungal species grown in Taiwan. A. cinnamomea has been reported to have many bioactivities, including anticancer and immunomodulating activities. However, the species is restricted to Cinnamomum kanehirai Hay (Lauraceae) and cannot be large-scale cultivated artificially until now. Consequently, it is becoming important to yield the fruiting bodies of “Chang-Zhi” by artificial cultivation. In our currently investigation, we have completed the establishment of two complementary DNA (cDNA) libraries from RNA isolated from liquid-cultured mycelia and fruit bodies of A. cinnamomea. Patterns of protein expression in liquid-cultured mycelia and fruit bodies of A. cinnamomea were investigated using two-dimensional polyacrylamide gel electrophoresis. According to our findings, it revealed that some identified proteins are related to lipids, amino acids and secondary metabolism in fruit body stage. The objective of this proposal is focused on identifying genes involved in the regulation of fruit body development by systems biology approach. In this study, the bioactive compounds that isolated from the extractives of wood of Cinnamomum kanehirai will be examined to realize the interaction between A. cinnamomea and the metabolites from C. kanehirai wood. Coupling with metabolomics, proteomics, microarray analysis, transformation system and bioinformatics, we hope in the future to explore the genes, which involved in the development of fruiting. We believe that the results obtained in this study would provide the useful technology, which will lead to the improvement of the fruiting of A. cinnamomea, protection the C. kanehirai tree, and drug discovery in the future.樟芝基因體學蛋白質體學代謝體學Antrodia cinnamomeagenomicsproteomicsmetabolomics