鄭石通臺灣大學:植物科學研究所林信宏Lin, Hsin-HungHsin-HungLin2010-05-112018-07-062010-05-112018-07-062009U0001-2207200913520400http://ntur.lib.ntu.edu.tw//handle/246246/181975The ipomoelin gene (IPO) was identified to be a wound inducible gene from sweet potato (Ipomoea batatas cv Tainung 57), and its expression was stimulated by ethylene, methyl jasmonate and hydrogen peroxide. IPO protein was also characterized as a defense related protein and it is a carbohydrate-binding protein. Recently, carbon monoxide (CO) was reported to play an important role in stress response and development, such as oxidative stress, salt stress, and root formation, in plants. The role of CO involved in wounding response has been further studied here. In the present of CO, the wound-induced IPO expression was inhibited. Heme oxygenase, which is a CO producing enzyme, was regulated in sweet potato to control IPO expression. CO induced the phosphorylation of p38-like MAPK in sweet potato, and negatively regulated the wound-induced IPO expression. Furthermore, the increase of cytosolic Ca2+ and the production of hydrogen peroxide were previously reported to be the main signal transducer inducing IPO expression. We found CO inhibited the accumulation of cytosolic Ca2+ stimulated by mechanical wounding. Besides, the production of hydrogen peroxide induced by wounding was also inhibited by catalase induced by CO. Finally, IPO gene is activated by dephosphorylated protein(s) via protein phosphatase, and that the position of dephosphorylated protein(s) is located in the very downstream of the signal transduction pathway for IPO expression.Contents bbreviations ------------------------------------------- 01hapter one: Literature review -------------------------- 03he roles of MJ signaling pathway involved in the of plant defense response -----------------------------------------04he roles of ethylene signaling pathway in the plant defense responses ----------------------------------------05APK cascades are involved in defense signaling ----------07PO signaling pathways and protein functions in sweet potato ---------------------------------------------------08EFERENCES ---------------------------------------------- 10hapter two: Calcium influxes and mitogen-activated protein kinase kinase activation mediate ethylene inducing ipomoelin gene expression in sweet potato --------------- 15BSTRACT -------------------------------------------------15NTRODUCTION ---------------------------------------------16ATERIALS AND METHODS ------------------------------------19lant materials and assay conditions ---------------------19NA isolation and analysis -------------------------------20a2+ detection by confocal scanning microscope -----------21ESULTS --------------------------------------------------23nduction ethylene on the accumulation of cytosolic Ca2+--23ffects of EGTA on the accumulation of cytosolic Ca2+ ----23ffects of EGTA on IPO expression ------------------------24ISCUSSION -----------------------------------------------26IGURES --------------------------------------------------30EFERENCES -----------------------------------------------35hapter three: Involvement of Carbon Monoxide in regulation of ipomoelin gene in sweet potato ------------------------39BSTRACT -------------------------------------------------39NTRODUCTION ---------------------------------------------40ATERIALS AND METHODS ------------------------------------46lant materials and assay conditions ---------------------46NA isolation and northern blot analysis -----------------462O2 detection by DAB staining ---------------------------472O2 content assay ---------------------------------------48a2+ detection by confocal scanning microscope -----------48estern blot analysis ------------------------------------49atalase activity staining -------------------------------50ESULTS --------------------------------------------------51O inhibits wounding-induced IPO expression---------------51nfluence of the heme oxygenase regulators in the expression of IPO ----------------------------------------52ffects of CO and p38 MAPK phosphorylation inhibitor on p38-like MAPK phosphorylation and IPO expression -------------53he involvement of CO in IPO signal transduction pathway inducing IPO expression ----------------------------------54O inhibits the accumulation of the cytosolic Ca2+ induced by mechanical wounding -----------------------------------55O inhibits the production of wounding-induced hydrogen peroxide through catalase --------------------------------56ffects of MAPK inhibitors and a protein phosphatase inhibitor on the IPO gene expression regulation of H2O2 induced --------------------------------------------------58ISCUSSION -----------------------------------------------60IGURES --------------------------------------------------67EFERENCES ----------------------------------------------81application/pdf2423069 bytesapplication/pdfen-US甘藷一氧化碳鈣離子傷害誘導基因calcium ioncarbon monoxidewound-inducible geneipomoelinsweet potatohttp://ntur.lib.ntu.edu.tw/bitstream/246246/181975/1/ntu-98-D92b42004-1.pdf