2008-01-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/663922摘要：防治植物病害之研究工作當中，發現施用水楊酸及樸殺熱可以降低百合感染灰黴病的嚴重度，在選殖水楊酸誘導之互補核酸中，鑑定一解碼富含甘胺酸蛋白質之互補核酸LsGRP1，其基因表現為水楊酸、樸殺熱誘導性，且為病原誘導性。由相似蛋白質之研究推測LsGRP1之表現與植物抗病性有關，期獲得LsGRP1之啟動子，用以分析基因表現模式及供分子抗病育種使用。本年度將選殖LsGRP1基因啟動子全長序列，完成順式因子之預測分析，以葵百合原生質體/PEG短暫表現系統分析不同處理下LsGPP1基因啟動子之表現情形，以瞭解LsGPP1之調控方式及其啟動子之誘導特性。<br> Abstract: In the study of application of induced resistance in plant disease control, we demonstrated that salicylic acid and probanazole could be used to reduce disease severity of Botrytis elliptica infection in lily. LsGRP1 cDNA has been cloned from Lilium `Star Gazer`and the Northern blot analysis demonstrated that the amounts of transcripts of LsGRP1 were significantly increased in the leaves of lily plants that were treated with SA and probenazole. Moreover, the transcripts of LsGRP1 were significantly increased in the lily leaves infected by Botrytis elliptica. Based on the protein百合富含甘胺酸蛋白質啟動子分子選殖LsGRP1promotermolecular cloning