2007-01-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/682424摘要：大波斯菊(Cosmos bipinnatus CAV.)，為菊科波斯菊屬植物，近年來因台灣農業的轉型，其已成為重要的景觀綠肥植物。民國93年底，首先於花蓮縣光復鄉，其後於花蓮縣其他各鄉鎮，發現具白色病斑病徵的大波斯菊病株。根據大波斯菊病害文獻的研究顯示，在花蓮地區之病害，疑似為日本及歐美地區已記載報導之大波斯菊白斑病，或稱白色黑穗病(white smut)，而本病害過去在台灣並未有正式之記載。本計畫之目的，乃在確認台灣地區本病害之發生情形，針對種子樣品發展快速篩選偵測技術，並對其發病生態及流行病學進行瞭解。本年度，將進一步調查此病害之田間發生情形，並收集不同來源或品種之大波斯菊種子，以及田間疑似罹病株樣品，利用Entyloma屬真菌ITS區域專一性PCR引子，結合Nested PCR的方法，專一且有效地偵測病原菌，並建立標準操作流程。<br> Abstract: Cosmos (Cosmos bipinnatus CAV.), belonging to Asteraceae, has recently become one of the important green manure recreation plant in Taiwan. In December 2004, symptomatic cosmos plants with whitish spots on the leaves were discovered in Guangfu Township of Hualien County, and the disease subsequently was also found in the surrounding areas. A similar disease called cosmos white smut was previously reported in Japan and Europe; however, it has not been officially documented in Taiwan. The goal of the project is to investigate the occurrence of cosmos white smut in Taiwan, develop the fast PCR detection technique for screening plant samples including seeds, and also study the ecology and epidemiology of the disease. In this year, we will further investigate the occurrence of this disease in Taiwan. Seed samples from different sources or varieties, and suspected diseased plants will be collected. A standard PCR detection protocol using the designed Entyloma ITS specific primers and nested PCR technique will be applied for screening. To the end, we hope that prevention of the disease spread and also better management strategies can be achieved based on proper diagnosis.大波斯菊白斑病PCR偵測技術CosmosWhite smutPCR detection technique