2005-01-012024-05-14https://scholars.lib.ntu.edu.tw/handle/123456789/659093摘要：可培養出較低成本之含多不飽和脂肪酸海洋性真菌Schizochytrium limacinum SR- 21，以生產成本合理之含DHA生物製品，供進一步飼養試驗評估該製品之功能性 。添加含DHA(二十二碳六烯酸) 菌藻製品於上市前肉雞飼料，提供便宜之DHA來 源，應可增進肌肉及肝臟中DHA含量。改善肉中DHA含量，可提升肉品之利用價 值。冀能藉由調控肝臟中參與脂肪生成基因的表現，改善家禽肝臟中脂肪代謝 ，減少血液中三酸甘油酯及膽固醇的濃度。所以藉由已建立簡易之產多不飽和脂 肪酸單細胞生物的培養方法，以進一步評估降低產製成本與利用這些產品於家禽 飼料中，應能提升肉品價值。可培養出較低成本之含多不飽和脂肪酸海洋性真菌 Schizochytrium limacinum SR-21，以生產成本合理之含DHA生物製品，供進一步 飼養試驗評估該製品之功能性。添加含DHA(二十二碳六烯酸) 菌藻製品於上市前 肉雞飼料，提供便宜之DHA來源，應可增進肌肉及肝臟中DHA含量。改善肉中 DHA含量，可提升肉品之利用價值。冀能藉由調控肝臟中參與脂肪生成基因的表 現，改善家禽肝臟中脂肪代<br> Abstract: Marine fungus, Schizochytrium limacinum SR-21, has potential to be a resource of docosahexaenoic acid ( DHA) production. The DHA content of total fatty acids of the strain was 32.7％. Utilization of bioreactor to culture the fungus can generate a DHAenriched fungal meal. At the beginning of the experiment, we will apply the optimal cultural conditions to mass culture and collect these microorganisms for broiler feeding test. We will study the optimal condition for a more economical way to produce the bioproduct. The feeding test includes 3 treatment batches: level of 0, 1, 3％ bioproducts will be added to the diets for 3 weeks. The 3-day-old broilers will feed the control diet ( bio-products or DHA oil not added ) for 2 weeks before examinating the feeding test (3 weeks). The second feeding trial is to feed the chickens with the bioproduct for 3 weeks and regular diet for 2 more weeks to study the carry over effect. At the end of the experiment, liver and muscle will be collected to determine the fatty acid composition and the expression of lipogenesis genes. We will also determine the contents of cholesterol and lipid in blood and tissue. Dietary supplementation of these DHA - enriched bio-products will increase the contents of DHA in broiler muscle and liver. By the basis of these established cultural methods of some microorganisms that can produce high PUFA, we can further estimate the practicability of these bio-products in poultry diets. It will reduct the feeding costs and enhance the value of meat products.二十二碳六烯酸多不飽和脂肪酸雞DHApolyunsaturated fatty acidchicken