Yuan HTsai T.-TWang H.-PChien Y.-SChen C.-AChu C.-CHo C.-TChu P.-HCHIEN-FU CHEN2021-08-052021-08-05202113858947https://www.scopus.com/inward/record.uri?eid=2-s2.0-85098193103&doi=10.1016%2fj.cej.2020.128131&partnerID=40&md5=6cb6b00aebed95127f5b0b8cb730c46fhttps://scholars.lib.ntu.edu.tw/handle/123456789/577061Myocardial infarction (MI) causes hundreds of thousands of deaths annually. Early diagnosis of MI by detecting the concentration of troponin I in plasma can help limit mortality rates. However, plasma separation and early diagnosis are particularly difficult to achieve in resource-limited settings. In this study, we demonstrate a manual and portable centrifuge along with a paper analytical device to measure troponin I for early MI diagnosis. With this so-called “Handfuge,” pure plasma can be achieved with a yield of up to 47.1% and purity as high as 99.9% in less than 3 min without electricity. The resulting plasma can then be directly used for detecting troponin I using a paper-based enzyme-linked immunosorbent assay (ELISA), with a detection limit as low as 0.03 ng/mL. The whole detection process consumes just 10 ?L of blood, which is similar to the volume obtained by a clinical finger-prick. The proposed centrifuge together with the paper analytical device possesses the potential to replace existing techniques in troponin I detection for early MI diagnoses in resource-limited regions of the world. ? 2020 Elsevier B.V.Analytic equipment; Cardiology; Diagnosis; Detection limits; Detection process; Early diagnosis; Enzyme linked immunosorbent assay; Mi diagnosis; Mortality rate; Myocardial Infarction; Plasma separation; Centrifuges[SDGs]SDG3journal article10.1016/j.cej.2020.1281312-s2.0-85098193103