2004-01-012024-05-13https://scholars.lib.ntu.edu.tw/handle/123456789/646079摘要：番茄因它的營養價值與對人類攝護腺癌的防護功能而大受歡迎。然而，在熱帶地 區，番茄常罹患一些疾病，如萎凋病、輪點病、番茄黃葉枯病與灰葉病。因此尋 找可能的方法以克服這些疾病成為當務之急。本計畫擬將阿拉伯芥 FIN219（AtFIN219）與番茄FIN219（LeFIN219）轉殖入番茄中，以獲得抗病性 的轉殖株。AtFIN219已被證實參與光敏素A導引的光訊息傳遞，並且受荷爾蒙 auxin與jasmonate的誘導表現；其中jasmonate已知是可以調控植物的防衛系統。近 來AtFIN219被發現是JAR1的等位基因，JAR1是參與jasmonate的訊息傳遞。利用 yeast two-hybrid篩選的方法，我們已獲得一個可與AtFIN219的C端相互作用的蛋白 質FIP1，它是一個可產生102氨基酸的蛋白質glutathione-S-transferase (GST)。 GST已被證實具有對殺草劑、病原菌解毒的功能。而且經由Northern Blot分析的 結果顯示：FIP1亦可受2,4-D與jasmonate的誘導。暗示AtFIN219是一個受光調控、 參與<br> Abstract: Popularity of Tomatoes is based on the nutrition and the medicinal value of protections of the prostate cancer in human. However, tomato, especially in tropical area, is suffering from some diseases such as bacterial wilt, bacterial spot, tomato yellow leaf curl virus (TYLCV), fusarium wilt, and gray leaf spot. Looking for the strategies to cure the disease effectively becomes important. The project is to introduce Arabidopsis FIN219(AtFIN219) and tomato FIN219(LeFIN219) into tomato, hoping to obtain disease-resistant transgenic tomatoes. AtFIN219 has been shown to be involved in phytochrome A-mediated light signaling pathway, and inducible by auxin and jasmonate, which is well documented to play important roles in plant defensive system. Recently, AtFIN219 is found to be allelic to JAR1, which is involved in jasmonate signaling pathway. By yeast two-hybrid screening, an AtFIN219-interacting protein FIP1 was recovered and demonstrated to interact with the C-terminal region of AtFIN219. FIP1 encodes a 102 amino-acid glutathione-S-transferase, which is involved in detoxification of herbicides and pathogens. Furthermore, FIP1 is also induced by 2,4-D and jasmonate based on the results of Northern Blot assays, suggesting that AtFIN219 is a light-regulated component involved in plant defense and plant growth and development. In addition, LeFIN219 has a 68% identity with AtFIN219 and its message is found to be present in a cDNA library constructed from elicitor-infected samples, implying that LeFIN219 is inducible by pathogens. So selection of disease resistant transgenic tomatoes mediated by LeFIN219 or AtFIN219 is feasible and promising.FIN219番茄抗病性茉莉酸FIN219disease resistance in tomatoesJasmonate