Li T.-Y.BEEN-HUANG CHIANG2019-07-112019-07-11201907533322https://scholars.lib.ntu.edu.tw/handle/123456789/413272The functions of 4-acetylantroquinonol B (4-AAQB), a ubiquinone derivative isolated from the mycelium of Antrodia cinnamomea, in immunotherapy for liver cancer were investigated. We found that 4-AAQB could inhibit liver cancer stem cell related manifestations and activate the antitumor ability of dendritic cells. Specifically, 4-AAQB can inhibit EpCAM, AFP and related pathways of HepG2 cells. It also significantly decreases the expression of £]-catenin, inhibits the tumorigenicity and decreases the secretion of immune escape related cytokines. Moreover, 4-AAQB can stimulate the proliferation of immune cells and promote the endocytosis of immature dendritic cells. When co-cultured immature dendritic cells with EpCAM+ HepG2 cells, 4-AAQB enhanced the expression of MHC class I and II on the surface of liver cancer stem cells and dendritic cells, increased the expression of costimulatory molecules CD80 of dendritic cells and cytokines related to immune activation. In conclusion, 4-AAQB from Antrodia cinnamomea can enhance immune function of dendritic cells against liver cancer stem cells, and may have the potential to be used for liver cancer prevention and immunotherapy. ? 20184-AAQBAFPAntrodia cinnamomeaDendritic cellsEpCAMLiver cancer stem cell[SDGs]SDG34 acetylantroquinonol b; antineoplastic agent; B7 antigen; beta catenin; epithelial cell adhesion molecule; fluorouracil; gamma interferon; interleukin 10; interleukin 6; tumor antigen; tumor necrosis factor; ubiquinone derivative; unclassified drug; 4-acetylantroquinonol B; cyclohexanone derivative; gamma butyrolactone; animal cell; antigen presentation; antineoplastic activity; Antrodia camphorata; Article; cancer immunotherapy; cancer prevention; cancer stem cell; carcinogenesis; CD4+ T lymphocyte; cell activation; cell function; cell maturation; cell subpopulation; cell viability; comparative study; confocal microscopy; controlled study; cytokine release; cytotoxicity; dendritic cell; Hep-G2 cell line; human; human cell; immune response; immunocompetent cell; immunoreactivity; liver cancer; nonhuman; priority journal; protein expression; rat; THP-1 cell line; analogs and derivatives; animal; Antrodia; cancer stem cell; cell survival; cellular immunity; coculture; dendritic cell; dose response; drug effect; immunology; isolation and purification; liver tumor; mouse; RAW 264.7 cell line; 4-Butyrolactone; Animals; Antrodia; Cell Survival; Coculture Techniques; Cyclohexanones; Dendritic Cells; Dose-Response Relationship, Drug; Hep G2 Cells; Humans; Immunity, Cellular; Liver Neoplasms; Mice; Neoplastic Stem Cells; RAW 264.7 Cellsjournal article10.1016/j.biopha.2018.11.101https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057288081&doi=10.1016%2fj.biopha.2018.11.101&partnerID=40&md5=93a1fe7c445f5197609213d59eedbd6c2-s2.0-85057288081https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057288081&doi=10.1016%2fj.biopha.2018.11.101&partnerID=40&md5=93a1fe7c445f5197609213d59eedbd6c