陳力騏臺灣大學：生物產業機電工程學研究所施炫合Shih, Syuan-HeSyuan-HeShih2007-11-262018-07-102007-11-262018-07-102007http://ntur.lib.ntu.edu.tw//handle/246246/52854本研究旨在建構一套適合於顯微鏡下自由操控生物高分子或細胞的經濟型掃瞄探針系統，其組成包括自製微電極、3D掃描系統與其使用者介面，以及可供直接觀察用的介電泳槽。 微電極的製作利用直徑0.5mm的鉛筆芯以電化學的方式於3Vrms交流電壓於1M NaOH溶液蝕刻9分鐘，並將蝕刻完的鉛筆芯以快速硬化劑將鉛筆芯封裝於玻璃毛細管中。最後再以鑽石砂紙研磨使微電極具微米大小的電活性區域露出(半徑約10μm)。以此方式製作的微電極的電化學特性近似於一般黏土碳微盤形電極。 3D掃描系統利用步進馬達以LabVIEWAn economic scanning probe system has been constructed for manipulating biomolecules and/or cells under microscopic observation; the system included a home-made microelectrodes, PC-controlled 3D-scanning system, the user interface and a dielectrophoresis chamber for direct observation. The micro-electrode was made by electrochemical etching (3Vrms for 9mins in 1M NaOH) of a pencil lead (0.5mm in diameter), and then the sharpened pencil lead was sealed (insulated) within a glass capillary using cyanoacrylate adhesive. The electrode tip was polished with diamond lapping films to reveal its micro-scaled electroactive region (~10μm in diameter). Its electrochemical characteristics are similar to a commercial carbon paste micro-disk electrode. The 3D-scanning system was driven with three stepped motors controlled by a user interface program written under Labview致謝........................................................i 中文摘要...................................................ii 英文摘要..................................................iii 目錄.......................................................iv 圖目錄....................................................vii 表目錄......................................................x 第一章 前言.................................................1 第二章 文獻探討.............................................3 2.1 微電極的發展........................................3 2.1.1 盤形微電極的製作....................................6 2.1.2 半球形微電極的製作..................................6 2.1.3 環形微電極的製作....................................7 2.1.4 環－盤形微電極的製作................................7 2.1.5 圓錐形微電極的製作..................................8 2.1.6 各式微電極的電化學特性..............................9 2.2 自動鉛筆芯電極的發展與應用.........................10 2.3 介電泳的發展.......................................11 2.4 本研究擬開發的系統架構.............................20 第三章 實驗材料與方法..................................21 3.1 實驗藥品與材料與儀器設備...........................21 3.1.1 實驗材料與裝置.....................................21 3.1.2 實驗藥品...........................................21 3.1.3 實驗儀器設備.......................................22 3.1.4 自製實驗器材.......................................23 3.1.5 實驗溶液製備.......................................25 3.2 實驗方法...........................................26 3.2.1 自動鉛筆芯電阻測試.................................26 3.2.2 自動鉛筆芯電化學蝕刻實驗...........................26 3.2.3 微鉛筆芯封裝實驗與微鉛筆芯電極表面研磨.............26 3.2.3.1 熱融micropipette tip式封裝.........................26 3.2.3.2 電化學電聚合高分子絕緣層封裝技術...................26 3.2.3.3 快速硬化劑填充玻璃毛細管封裝技術...................28 3.2.3.4 封裝後微鉛筆芯電極表面研磨.........................28 3.2.3.5 微電極性能測試.....................................28 3.2.4 掃瞄式微探針系統應用於介電泳實驗...................30 3.2.4.1 Latex介電泳實驗....................................30 3.2.4.2 紅血球介電泳實驗...................................30 第四章 實驗結果與討論..................................32 4.1 Pentel C205系列與Pilot ENO系列自動鉛筆芯導電度探討.34 4.2 自動鉛筆芯蝕刻參數探討.............................34 4.2.1 二鉻酸鉀溶液對自動鉛筆芯的蝕刻效果.................34 4.2.2 氫氧化鈉溶液對自動鉛筆芯的蝕刻效果.................36 4.2.3 田口法參數探討.....................................39 4.3 微鉛筆芯的封裝與研磨技術探討.......................41 4.3.1 熱融micropipette tip封裝的微鉛筆芯電極特性.........41 4.3.2 電化學電聚合絕緣層封裝技術探討與電極特性...........43 4.3.3 快速硬化劑填充玻璃毛細管封裝的微鉛筆芯電極特性.....46 4.3.4 微鉛筆芯電極製作技術綜合比較.......................46 4.4 介電泳實驗結果.....................................48 4.4.1 乳膠微粒介電泳實驗.................................48 4.4.2 紅血球介電泳實驗...................................48 4.4.3 介電泳實驗探討.....................................48 第五章 結論與未來展望..................................51 參考文獻...................................................52 附錄A LabVIEW3713661 bytesapplication/pdfen-US微鉛筆芯電極鉛筆芯蝕刻紅血球細胞介電泳操控經濟型細胞分子操控平台MicroelectrodesDielectrophoresisCell manipulationIndium tin oxidethesishttp://ntur.lib.ntu.edu.tw/bitstream/246246/52854/1/ntu-96-R94631015-1.pdf