王一中2006-07-262018-07-122006-07-262018-07-122003http://ntur.lib.ntu.edu.tw//handle/246246/26729Pterygium is a worldwide ocular surface disease, an ocular disorder that occurs more often in the population of tropical and subtropical region 1. It often causes irritating or inflamed ocular symptoms, and also accompanies with local drying of corneas and conjunctivas. Elastin is a major component of connective tissue in large arteries, lung, skin, and ligmenetum, and provides elasticity in these tissues 2. In normal skin, elastic fibers are found in the dermal connective tissue in only small amounts. In photodamaged skin, on the other hand, elastic fibers are the most prominent component of extracellular matrix and are located in the superficial to mid-dermis as an amorphous blue staining area on routine hematoxylin-eosin staining 3,4 Accumulation of elastin in solar elastosis of photodamaged skin has also been demonstrated in clinical situation and experimental animals, by both immunohistochemistry and molecular biology techniques5,6. It is likely that the pathologic changes in conjunctiva in response to chronic UV irradiation are similar to those in chronically sun-damaged skin. For example, elastodysplasia and elastodystrophy are known to be present in the subepithelial connective tissue of pinguecular part in pterygium, and similar pathologic elastin accumulation is found in chronically sun-damaged skin 7. Therefore,we speculate that the pathogenesis of pterygia may be the same as photodamaged skin. The increase in elastin mRNA levels has been demonstrated in fibroblasts of photoaged skin, as compared with those from skin of normal subjects. This increased level of elastin mRNA in sun-damaged skin is resulted from enhanced elastin promoter activity. Although we found that post-transcriptional regulation of elastin promoter plays an important role in the formation of elastoid degeneration of pterygium, we still want to investigate the possibility of transcriptional regulation of elastin promoter. We will try to use transgenic model to study the elastin expression in pterygium.8 In the present study, we tried to generate a transgenic mice carry a 2.8 kb human elastin promoter with a GFP reporter gene, and apply this mice to the model of pterygial formation. We also try to generate an ablation model of conjunctival fibroblasts by using DTA constructs.application/pdf130308 bytesapplication/pdfzh-TW國立臺灣大學醫學院眼科[SDGs]SDG3journal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/26729/1/912314B002190.pdf