Chang, K.-W.K.-W.ChangNIEN-TSU HUANG2021-05-052021-05-052019https://www.scopus.com/inward/record.url?eid=2-s2.0-85094971828&partnerID=40&md5=fc78b547273f8b20493867a076063e05https://scholars.lib.ntu.edu.tw/handle/123456789/559131The antibiotic susceptibility test (AST) is essential in the clinical diagnosis of serious bacterial infection such as sepsis. However, the whole AST usually takes prolonged time (2-5 days) for bacterial culture, antibiotic treatment and data analysis. To address this problem, we developed a microfluidic system integrating membrane filtration and SERS substrate (MF-SERS) to perform on-chip bacterial enrichment, metabolite collection, and in-situ SERS measurements. By using this system, bacteria and secreted metabolites are enriched in a well-confined microenvironment, which can minimize the required bacteria concentration and enable a rapid, uniform and sensitive AST readout for various infection disease diagnosis applications. © 2019 CBMS-0001.Antibiotic susceptibility test; Membrane filtration; Surface-enhanced Raman scattering (SERS)Antibiotics; Bacteria; Diagnosis; Fluidic devices; Metabolites; Microfiltration; Raman scattering; Substrates; Surface scattering; Surface testing; Antibiotic susceptibility tests; Antibiotic treatment; Bacteria concentrations; Bacterial infections; Clinical diagnosis; Membrane filtrations; Micro fluidic system; Surface enhanced Raman Scattering (SERS); Microfluidicsconference paper2-s2.0-85094971828