林讚標2006-07-262018-07-062006-07-262018-07-062004-07-31http://ntur.lib.ntu.edu.tw//handle/246246/17463萬年松（Selaginella tamariscina）是一種可以忍受極度缺水的復活植物。本 研究以萬年松為材料欲探討植物體在忍受乾旱逆境時的可能分子機制。利用 differential display 與5’-race 等分子工具篩選萬年松在缺水逆境的過程中受到誘 導表現的基因。結果收集了約100 個受缺水誘導表現的cDNA clone ，從其中18 個表現較強的clone 中得到12 個全長cDNA。經序列比對後，發現在其他植物皆 有相似的基因存在。目前正在進行northern blot 分析，確認這些cDNA 的差異表 現確實是受到乾旱的誘導。未來將進一步以chromosome walking 的策略得到這 些受到乾旱誘導基因的promoter 序列，分析其cis-acting element，以釐清萬年松 忍受乾旱的分子機制。Selaginella tamariscina, a resurrection plant, can survive severe water stress. To investigate the mechanism of desiccation tolerance of resurrection plant, differential display was employed to find the dehydration induced genes in S. tamariscina during dehydration. About 100 dehydration induced cDNA clones were collected. Among them, the full length of 12 strongly induced cDNA clones were obtained by using 5’-race technology. These12 full-length cDNA clones were identified because high similarity to the orthologs in other plants. Northern blot analysis was also applied to conform that these cDNA clones were actually dehydration activated. In the future, chromosome walking will be executed to obtain the promoter sequence of these dehydration inducible genes, and the cis-acting element will be analyzed to elucidate the molecular mechanism of desiccation tolerance of S. tamariscina.application/pdf245463 bytesapplication/pdfzh-TW國立臺灣大學植物科學研究所journal articlehttp://ntur.lib.ntu.edu.tw/bitstream/246246/17463/1/922313B002041.pdf