Wang W.-K.CHI-TAI FANGChen H.-L.Yang C.-F.YEE-CHUN CHENChen M.-L.SHEY-YING CHENYang J.-Y.Lin J.-H.PAN-CHYR YANGSHAN-CHWEN CHANG2020-03-242020-03-2420050095-1137https://scholars.lib.ntu.edu.tw/handle/123456789/478751We examined severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA in plasma of 32 patients (probable SARS cases) by a quantitative real-time reverse transcription-PCR assay and reported that the highest detection rate, 75%, was found between day 5 and day 7 of illness, followed by rates of 64, 50, and 38% found between day 8 and day 11, day 2 and day 4, and day 12 and day 16, respectively. Analysis of sequential SARS-CoV load in plasma from sis cases revealed different patterns of viremia, with the peak between day 4 and day 8. Our findings of the high detection rate of SARS-CoV RNA in plasma before day 11, together with the relative convenience of collecting and handling plasma, suggest that plasma can be used for early diagnosis of SARS.[SDGs]SDG3virus RNA; adult; aged; article; chronology; clinical article; early diagnosis; female; human; male; plasma; priority journal; real time polymerase chain reaction; RNA isolation; severe acute respiratory syndrome; viremia; virus identification; virus load; Adult; Aged; Female; Humans; Male; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; SARS Virus; Severe Acute Respiratory Syndrome; Taiwan; Coronavirus; RNA viruses; SARS CoVjournal article10.1128/JCM.43.2.962-965.2005156957192-s2.0-13844266127