Han, Yu-SanYu-SanHanLiao, I-ChiuI-ChiuLiaoHuang, Yung-SenYung-SenHuangTzeng, Wann-NianWann-NianTzengYu, John Yuh-LinJohn Yuh-LinYu2008-04-102018-07-062008-04-102018-07-06200300166480http://ntur.lib.ntu.edu.tw//handle/246246/64333http://ntur.lib.ntu.edu.tw/bitstream/246246/64333/1/1004.pdfhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0042164475&doi=10.1016%2fS0016-6480%2803%2900143-6&partnerID=40&md5=dad7c2dae8535c3e59c793ce539c17bcThe complete complementary DNA (cDNA) encoding pituitary gonadotropin II-β subunit (GTH II-β) of Japanese eel Anguilla japonica was cloned and sequenced, and the profiles of pituitary glycoprotein hormone α subunit (PGH-α), GTH I-β, and GTH II-β mRNA transcript levels at different stages of ovarian development before vitellogenesis in the wild females were investigated. The maturity of female eels was divided into four stages: juvenile, sub-adult, pre-silver, and silver stages based on ovarian development and skin color. The GTH II-β cDNA was cloned by reverse transcription and polymerase chain reaction (RT-PCR) amplification from total pituitary RNA. The full length GTH II-β cDNA was obtained using 5′- and 3′-rapid amplification of cDNA ends. The cloned eel GTH II-β cDNA consists of 646bp nucleotides, including 53bp nucleotides of 5′-untranslated region (UTR), 423bp of open reading frame, and 170bp nucleotides of 3′-UTR followed by a poly(A) tail. It encodes a 140-amino acid precursor molecule of GTH II-β subunit with a putative signal peptide of 24 amino acids and a mature peptide of 116 amino acids. RT-PCR analysis showed that the pituitary transcript levels of α subunit steadily increased during eel silvering. The expression of GTH I-β and II-β mRNA levels, however, varied in different ovarian developmental stages. The mRNA expression of both GTH I-β and GTH II-β were detectable in juvenile stage. The expression levels of GTH II-β mRNA, but not GTH I-β, were significantly increased in sub-adult stage. The transcript levels of GTH I-β and II-β subunits further increased in pre-silver and silver stages. We demonstrated for the first time that the differential transcription patterns of pituitary PGH-α, GTH I-β, and GTH II-β mRNAs occur during silvering of the wild female Japanese eels. © 2003 Elsevier Science (USA). All rights reserved.en-USJapanese eel (Anguilla japonica)silveringovarian developmentGTH I-β subunitGTH II-β subunitα subunitgene expressionGene expression; GTH I-β subunit; GTH II-β subunit; Japanese eel (Anguilla japonica); Ovarian development; Silvering; α-Subunit[SDGs]SDG14amino acid; complementary DNA; gonadotrophin 1 beta; gonadotrophin 2 beta; gonadotropin; hypophysis hormone; messenger RNA; nucleotide; pituitary glycoprotein hormone alpha; RNA; signal peptide; unclassified drug; 3' untranslated region; 5' untranslated region; alpha chain; anguilla japonica; animal cell; animal tissue; article; beta chain; developmental stage; eel; female; gene amplification; gene expression; genetic code; genetic transcription; juvenile; maturation; molecular cloning; molecule; nonhuman; nucleotide sequence; open reading frame; ovary development; precursor; priority journal; reverse transcription polymerase chain reaction; skin color; vitellogenesisjournal article10.1016/S0016-6480(03)00143-6128998422-s2.0-0042164475http://ntur.lib.ntu.edu.tw/bitstream/246246/64333/1/1004.pdf