Chou Y.-SLin Y.-CTai-Horng YoungPEI-JEN LOU2020-10-272020-10-2720161043-3074https://www.scopus.com/inward/record.uri?eid=2-s2.0-84933544731&doi=10.1002%2fhed.23986&partnerID=40&md5=cff0e2d50a86ce5e81f20a53320bf3cehttps://scholars.lib.ntu.edu.tw/handle/123456789/518210Background Artificial salivary gland replacement would be an ideal treatment for xerostomia. In vivo, salivary gland cells are surrounded by a complex stromal environment in which fibroblasts are the main cell type in proximity to the gland cells. However, very little is known about the relationship between these fibroblasts and the gland cells. Methods Parotid gland acinar cells (PGACs) and fibroblasts from the same human gland were cocultured. PGAC function-related protein expression was investigated. Results The expression of α-amylase in PGACs was increased in a fibroblast ratio-dependent manner. Both fibroblast-conditioned medium and direct coculture also significantly enhanced the PGAC expression of α-amylase. Basic fibroblast growth factor (bFGF) seems to be a regulator of α-amylase expression in PGACs. Conclusion An appropriate number of fibroblasts in contact with the PGACs is necessary to promote PGAC function. Fibroblast-secreted bFGF may play a paracrine signaling role in the regulation of α-amylase expression in PGACs. ? 2015 Wiley Periodicals, Inc..[SDGs]SDG3amylase; fibroblast growth factor 2; amylase; fibroblast growth factor 2; acinar cell; Article; cancer surgery; cell differentiation; cell lysate; cell proliferation; clinical article; coculture; controlled study; fibroblast; human; paracrine signaling; parotid gland; parotid gland tumor; priority journal; protein expression; acinar cell; cell culture; cytology; fibroblast; metabolism; parotid gland; Acinar Cells; alpha-Amylases; Cells, Cultured; Coculture Techniques; Fibroblast Growth Factor 2; Fibroblasts; Humans; Parotid Glandjournal article10.1002/hed.23986255453532-s2.0-84933544731