CHING-HUA KUOLee C.-W.Lin S.-C.Tsai I.-L.SHOEI-SHENG LEEYUFENG JANE TSENGKang J.-J.Peng F.-C.Wei-Chu L.2021-06-042021-06-042010399140https://www.scopus.com/inward/record.uri?eid=2-s2.0-75749114019&doi=10.1016%2fj.talanta.2009.10.003&partnerID=40&md5=eedf3b1af953e83fb3ba81d187340b73https://scholars.lib.ntu.edu.tw/handle/123456789/565248Aristolochic acids (AAs) are a mixture of structural-related compounds, in which aristolochic acid I (AA I) and aristolochic acid II (AA II) are reported to be correlated with Aristolochic acid nephropathy (AAN). In this work, a rapid and sensitive ultra-high-pressure liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed to determine AA I and AA II in herbal products and biological fluids. By using gradient elution with a mobile phase composed of a mixture of 10 mM ammonium formate buffer (pH 3.0) and acetonitrile, AAs could be determined within 10 min. Under optimum UHPLC-MS/MS conditions, the limit of detections was 0.14 and 0.26 ng mL-1 for AA I and AA II, respectively. Run-to-run repeatability and intermediate precision of peak area for AA I and AA II were less than 5.74% relative standard deviation (RSD). Accuracy was tested by spiking 10, 100 and 1000 ng mL-1 in rat serum and the recoveries were within 76.5-92.9%. Matrix effects were within 78.8-127.7%. The developed method was successfully applied to determine AA I and AA II in several herbal products and to investigate their pharmacokinetic behavior in female Wister rats. The result shows that the developed UHPLC-MS/MS method is efficient, sensitive, and accurate for the determination of AA I and AA II in herbal products and biological samples. © 2009 Elsevier B.V. All rights reserved.Aristolochic acid; Aristolochic acid nephropathy; Biological samples; Herbal products; UHPLC-MS/MSBody fluids; High pressure liquid chromatography; Mass spectrometry; Mixtures; Aristolochic acid; Biological samples; Herbal products; Nephropathy; U-HPLC-MS; Ascorbic acidjournal article10.1016/j.talanta.2009.10.003201523952-s2.0-75749114019