國立臺灣大學植物病理與微生物學系暨研究所石正人2006-07-262018-06-292006-07-262018-06-291999http://ntur.lib.ntu.edu.tw//handle/246246/17668本計畫利用斜紋夜蛾細胞株SL7B 進行細胞培養液改良試驗以進行病毒 量產試驗，發現就細胞株種類而言，斜 紋夜蛾細胞株比現行商品化秋行軍蟲 細胞株SF21AE 更適合用來生產苜蓿 夜蛾核多角體病毒。利用簡易培養液 ISC-03 培養SL7B 細胞時，隨培養液中 添加血清濃度增加，細胞生長速度加快 且細胞生產野生型病毒數量與表現重 組病毒外源蛋白產量均增加。Pluronic F-68 添加物對細胞生長有益，在含2% 胎牛血清的ISC-03 培養液中添加0.1% 的Pluronic F-68，則細胞生長速度比添 加8%胎牛血清者更快；當培養液中血 清濃度相同時，Pluronic F-68 的添加不 但可增加細胞生產病毒核多角體數量 且可提高重組蛋白螢光酵素表現量。The imprivement of media for culturing cells from Spodoptera litura. Cheng-Jen Shih, Department of Entomology, National Taiwan University A cell line derived from Spodoptera litura, SL7B, was used to ascertain for the improvement of media for mass production of Autograph californica nucleopolyhedrovirus (AcMNPV). Results revealed that SL7B cell line was better than commercial cell line, SF21AE, for the replication of wild type AcMNPV and recombinant virus protein expressed. SL7B cells can grow in low cost and simple ISC-03 medium, the speed of cell growth was increased upon the increase of concentration of fetal bovine serum (FBS) contained in cultural medium. In addition, the production of wild type AcMNPV and amount of recombinant protein luciferase expressed had positive correlation to the concentration of FBS in medium. ISC-03 medium supplemented with Pluronic F68 was helpful to the growth of SL7B cells. When Pluronic F-68 added to the medium containing 2% FBS, the growth of cell was faster than the medium containing 8% FBS. Moreover, in the medium containing same concentration of FBS, both of wild type AcMNPV and recombinant protein production were much higher when Pluronic F-68 was supplemented.application/pdf27167 bytesapplication/pdfzh-TW國立臺灣大學植物病理與微生物學系暨研究所斜紋夜蛾細胞株苜蓿夜蛾核多角體病毒重組蛋白產量細胞倍增時間細胞培養液添加物Spodoptera lituracell lineAcMNPVrecombinant protein expressioncell population doubling timemedium supplementsotherhttp://ntur.lib.ntu.edu.tw/bitstream/246246/17668/1/882313B002049.pdf