YA-CHIEN YANGHsu T.-Y.Chen J.-Y.Yang C.-S.Lin R.-H.2020-06-192020-06-1920010007-1048https://scholars.lib.ntu.edu.tw/handle/123456789/503693Cord blood T cells are much more likely to be induced to apoptosis in vitro than adult T cells. Nevertheless, the expression of Fas is markedly lower on cord blood lymphocytes than on peripheral blood lymphocytes. In the current investigation, we determined the capacity of tumour necrosis factor-α (TNF-α) to induce apoptosis in human na?ve T cells in cord blood, and assessed the roles of two distinct TNF receptors (TNFRs) in mediating death signals. After activation, cord blood T cells were sensitive to TNF-α-induced apoptosis, and interleukin 2 (IL-2) could prevent this apoptotic response. Both TNFR1 (p55) and TNFR2 (p75) expressed on activated cord blood T cells were able to transmit apoptotic signals. Moreover, a synergistic effect was observed by a combination of TNFR1- and TNFR2-signals. Additionally, CD4+ T cells showed higher sensitivity to TNFR-mediated apoptosis than CD8+ T cells. These data suggest that TNF-α probably is a mediator of apoptosis in cord blood T cells in vivo and may contribute to the low incidence of graft-versus-host disease in cord blood transplantation.[SDGs]SDG3Fas antigen; interleukin 2; protein p55; protein p75; tumor necrosis factor alpha; tumor necrosis factor receptor 1; tumor necrosis factor receptor 2; antigen expression; apoptosis; article; controlled study; graft versus host reaction; human; human cell; peripheral lymphocyte; priority journal; signal transduction; T lymphocyte; T lymphocyte activation; transplantation; umbilical cord bloodjournal article10.1046/j.1365-2141.2001.03090.x117033472-s2.0-0035725753