MANUEL MAESTRE-REYNAWang, Po-HsunPo-HsunWangNango, ErikoErikoNangoHosokawa, YuheiYuheiHosokawaSaft, MartinMartinSaftFurrer, AntoniaAntoniaFurrerYang, Cheng-HanCheng-HanYangGusti Ngurah Putu, Eka PutraEka PutraGusti Ngurah PutuWu, Wen-JinWen-JinWuEmmerich, Hans-JoachimHans-JoachimEmmerichCaramello, NicolasNicolasCaramelloFranz-Badur, SophieSophieFranz-BadurYang, ChaoChaoYangEngilberge, SylvainSylvainEngilbergeWranik, MaximilianMaximilianWranikGlover, Hannah LouiseHannah LouiseGloverWeinert, TobiasTobiasWeinertWu, Hsiang-YiHsiang-YiWuLee, Cheng-ChungCheng-ChungLeeHuang, Wei-ChengWei-ChengHuangHuang, Kai-FaKai-FaHuangChang, Yao-KaiYao-KaiChangLiao, Jiahn-HaurJiahn-HaurLiaoWeng, Jui-HungJui-HungWengGad, WaelWaelGadChang, Chiung-WenChiung-WenChangPang, Allan HAllan HPangYang, Kai-ChunKai-ChunYangLin, Wei-TingWei-TingLinChang, Yu-ChenYu-ChenChangGashi, DardanDardanGashiBeale, EmmaEmmaBealeOzerov, DmitryDmitryOzerovNass, KarolKarolNassKnopp, GregorGregorKnoppJohnson, Philip J MPhilip J MJohnsonCirelli, ClaudioClaudioCirelliMilne, ChrisChrisMilneBacellar, CamilaCamilaBacellarSugahara, MichihiroMichihiroSugaharaOwada, ShigekiShigekiOwadaJoti, YasumasaYasumasaJotiYamashita, AyumiAyumiYamashitaTanaka, RieRieTanakaTanaka, TomoyukiTomoyukiTanakaLuo, FangjiaFangjiaLuoTono, KensukeKensukeTonoZarzycka, WiktoriaWiktoriaZarzyckaMüller, PavelPavelMüllerAlahmad, Maisa AlkhederMaisa AlkhederAlahmadBezold, FilippFilippBezoldFuchs, ValerieValerieFuchsGnau, PetraPetraGnauKiontke, StephanStephanKiontkeKorf, LukasLukasKorfReithofer, ViktoriaViktoriaReithoferRosner, Christian JoshuaChristian JoshuaRosnerSeiler, Elisa MarieElisa MarieSeilerWatad, MohamedMohamedWatadWerel, LauraLauraWerelSpadaccini, RobertaRobertaSpadacciniYamamoto, JunpeiJunpeiYamamotoIwata, SoSoIwataZhong, DongpingDongpingZhongStandfuss, JörgJörgStandfussRoyant, AntoineAntoineRoyantBessho, YoshitakaYoshitakaBesshoEssen, Lars-OliverLars-OliverEssenTsai, Ming-DawMing-DawTsai2023-12-272023-12-272023-120036-80751095-9203https://scholars.lib.ntu.edu.tw/handle/123456789/638155Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism of the photolyase-catalyzed repair of a cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. We used these results to create a movie that depicts the repair of CPD lesions in the picosecond-to-nanosecond range, followed by the recovery of the enzymatic moieties involved in catalysis, completing the formation of the fully reduced enzyme-product complex at 500 nanoseconds. Finally, back-flip intermediates of the thymine bases to reanneal the DNA were captured at 25 to 200 microseconds. Our data cover the complete molecular mechanism of a photolyase and, importantly, its chemistry and enzymatic catalysis at work across a wide timescale and at atomic resolution.en[SDGs]SDG2[SDGs]SDG13journal article10.1126/science.add7795380330542-s2.0-85178506269https://api.elsevier.com/content/abstract/scopus_id/85178506269