2015-06-012024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/670520摘要：本產學計畫主要在開發生物可降解聚胺酯作為醫療用高風險管類材料，而主 要之先導產品為可吸收型之人工小血管及神經導管。本計畫為將聚胺酯作為人工 血管與神經導管之材料，經過配方設計並以掃描式電子顯微鏡 (SEM)及萬能拉 力機評估，獲得最適化之孔隙結構及機械性質。為使人工血管性質更接近天然血 管，進一步將血管內表層以磺酸化幾丁聚醣改質，以降低血小板吸附量；外表層 則以浸塗法 (dip coating) ，浸塗一層厚約30 m的聚胺酯，防止滲血。我們製備 非對稱性孔洞結構之神經導管，並進行神經導管動物移植實驗，在實驗期間 (六 週) ，藉由步態分析評估再生神經肌肉功能性，並使用磁振造影(MRI) 及犧牲後 之組織切片觀察神經纖維再生情形，結果證實聚胺酯神經導管有助於周邊神經再 生面積可達0.23 mm2，此新材料較文獻中報導之傳統可分解高分子效果更好。以 上結果期望能發展聚胺酯生醫產業品，並拓展其量產，以扶植本土優良傳統產業 進行高附加價值醫療器材原料與產品之開發與製造。 關<br> Abstract: The project is to develop biodegradable polyurethane for class III high class medical devices. The main frontier product intended is the absorbable small-caliber vascular grafts and nerve conduits. This project employs polyurethane as biomaterials to fabricate vascular grafts and nerve conduits with optimized microporous structure and mechanical properties evaluated by SEM and stress-strain measurement. In order to reduce platelet adhesion and prevent the graft from bleeding, the inner surface was further modified by a negatively charged sulfonated chitosan following air plasma treatment. The outer layer was dip-coated with a 30- m-thin dense layer. We also fabricated nerve conduits with asymmetric microporosity and implanted in rats for in vivo evaluation. The walking track analysis was used to assess the functional recovery of the implanted animals. Nerve regeneration was visualized by a Bruker 7-T MRI instrument (Germany) and confirmed by histology after animal sacrifice. We demonstrated that the asymmetric microporous structure conduits were beneficial for sciatic nerve reconstruction (with a regenerated area of 0.23 mm2). We expect that our endeavor may bring the novel polyurethane into biomedical industry and that our collaborating company with a former reputation in traditional industry can extend its polyurethane product lines to biotechnology by offering the high added value medical class materials and devices. Keywords:可降解聚胺酯人工小血管神經導管Biodegradable polyurethanevascular graftsnerve conduits