Sun T.-P.Tai C.-H.Wu J.-T.Wu C.-Y.Liang W.-C.Chen H.-Y.2019-05-222019-05-22201620474830https://scholars.lib.ntu.edu.tw/handle/123456789/409848"Click" reactions provide precise and reliable chemical transformations for the preparation of functional architectures for biomaterials and biointerfaces. The emergence of a multiple-click reaction strategy has paved the way for a multifunctional microenvironment with orthogonality and precise multitasking that mimics nature. We demonstrate a multifaceted and route-controlled click interface using vapor-deposited functionalized poly-para-xylylenes. Distinctly clickable moieties of ethynyl and maleimide were introduced into poly-para-xylylenes in one step via a chemical vapor deposition (CVD) copolymerization process. The advanced interface coating allows for a double-click route with concurrent copper(i)-catalyzed Huisgen 1,3-dipolar cycloaddition (CuAAC) and the thiol-maleimide click reaction. Additionally, double-click reactions can also be performed in a cascade manner by controlling the initiation route to enable the CuAAC and/or thiol-yne reaction using a mono-functional alkyne-functionalized poly-para-xylylene. The use of multifaceted coatings to create straightforward and orthogonal interface properties with respect to protein adsorption and cell attachment is demonstrated and characterized. ? 2015 The Royal Society of Chemistry.[SDGs]SDG3journal article10.1039/c5bm00417a2-s2.0-84955580593https://www.scopus.com/inward/record.uri?eid=2-s2.0-84955580593&doi=10.1039%2fc5bm00417a&partnerID=40&md5=b8552118a490d99f4fc010b217741bcf