Chi, S.C.S.C.ChiLo, C.F.C.F.LoChang, P.S.P.S.ChangPeng, S.E.S.E.PengKou, G.H.G.H.KouSHAU-CHI CHI2009-07-152018-07-062009-07-152018-07-061997http://ntur.lib.ntu.edu.tw//handle/246246/162122Mass mortalities of hatchery-reared juvenile groupers have occurred in southern Taiwan. The diseased fish swam in a darting, corkscrew fashion. Light microscopy revealed vacuolation in the brain tissue. Electron microscopy showed numerous non-enveloped, cytoplasmic viral particles (20-25 nm in diameter) in the brain cells, and many virions were enclosed in the membrane-bound organelles of the cells. Two structural proteins of the purified grouper virus, with molecular weights of 44 and 43 kDa, were revealed by SDS-PAGE. Moreover, the results of RT-PCR and nested PCR diagnosis using primers specific to the T2 and T4 target segments of striped jack nervous necrosis virus (SJNNV) RNA2 genes suggest that this virus is a fish nodavirus, and is designated as GNNV 9410 strain (grouper nervous necrosis virus strain 9410). This is the first case report of viral nervous necrosis among marine fish in Taiwan.application/pdf992465 bytesapplication/pdfen-US[SDGs]SDG14Caranx delicatissimus; Epinephelus; Epinephelus akaara; Nodaviridae; Redspotted grouper nervous necrosis virus; Striped Jack nervous necrosis virus10.1046/j.1365-2761.1997.00291.x2-s2.0-0030976866http://ntur.lib.ntu.edu.tw/bitstream/246246/162122/1/03.pdf