Luo, X.L.X.L.LuoWu, H.C.H.C.WuTsao, C.Y.C.Y.TsaoCheng, Y.Y.ChengRubloff, G.W.G.W.RubloffBentley, W.E.W.E.BentleyHSUAN-CHEN WU2018-09-102018-09-102010http://www.scopus.com/inward/record.url?eid=2-s2.0-84884321397&partnerID=MN8TOARShttp://scholars.lib.ntu.edu.tw/handle/123456789/355628https://www.scopus.com/inward/record.uri?eid=2-s2.0-84884321397&partnerID=40&md5=a1b96477c6dbb7050cf5ca25093297efWe demonstrate the biofabrication of three dimensional (3D) biopolymer membranes and the assembly of two cell lines in a micro-sandwich structure in microfluidics. A freestanding chitosan membrane was first fabricated by using pH gradients generated at the flow interface of two converging flows. The micro-sandwich was then fabricated by cross-linking alginate on both sides of the chitosan membrane with diffusion of calcium ions through the semi-permeable chitosan membrane. Cell assembly was achieved by blending cells into the alginate solution to embed the target cells into the micro-sandwich alginate scaffolds. The cell assembly process is simple, fast and easy to control.Biofabrication; Cell assembly; Membrane; Three dimensionalAlginate scaffolds; Alginate solutions; Biofabrication; Cell assembly; Chitosan membrane; Converging flow; Flow interface; Target cells; Alginate; Biomolecules; Blending; Cell culture; Cells; Chitosan; Membranes; Microfluidics; Three dimensional; Scaffolds (biology)conference paper2-s2.0-84884321397