2009-08-012024-05-15https://scholars.lib.ntu.edu.tw/handle/123456789/661849摘要：蝴蝶蘭為世界性之重要花卉作物，近年來已成為荷蘭拍賣市場交易量與交易總值第一名之盆花。蝴蝶蘭為臺灣重要之外銷商品，每年外銷產值與產量均有大量而快速的成長，產業亦逐漸走向大規模栽培與企業化管理之型態。欲提升生產之經濟效益與商品品質，須對蝴蝶蘭各個生產階段的肥培管理有更精準的調控。前人對蝴蝶蘭氮素營養之相關研究，多針對組織內氮素濃度分析，及肥料中氮素濃度對其生長之影響作探討，尚無文獻指出蝴蝶蘭於不同生長時期對氮素營養需求之差異。另一方面，蝴蝶蘭對肥料反應遲緩，可能係因根葉具有貯藏的功能。利用傳統肥料試驗與營養分析方法，無法得知蝴蝶蘭不同器官及不同生長階段對養分的精確需求，亦無法探究其對氮素的吸收與分配。 15N是穩定的氮同位素，人為施予植物經15N標定的氮肥，可研究植物對氮的吸收，並可區分植體中的氮素來自儲存氮或肥料氮。因不具放射性，15N為一對環境與研究人員安全的研究方法。本計畫將利用15N作為氮肥之示蹤原子，研究蝴蝶蘭不同生長階段及不同肥料狀態下，對於氮素之吸收與分配、各個器官於不同時期對氮素之積儲強度與其是否扮演供源之角色、根葉對氮素吸收效率之差異以及花梗發育之氮素來源。進而詳細探討蝴蝶蘭在營養與生殖生長時期，對於氮素之吸收與利用、各器官做為供源或積儲之相互關係。期望藉由本計畫蝴蝶蘭氮素營養代謝的基礎研究，改善產業之肥培管理技術，提升蝴蝶蘭植株品質與商品價值，同時供學術及產業之利用。 <br> Abstract: Phalaenopsis is a floricultural crop with worldwide importance. It ranks the top one potted flower in trade value and in quantity of FloraHolland Auction. Phalaenopsis is the most important exported floral product of Taiwan. The exporting value and quantity increase greatly and quickly in the recent years. The Phalaenopsis industry in Taiwan is going on a trend of large scale production. In order to increase the production efficiency and quality, precise control of every producing stage of Phalaenopsis is a must. Former related researches on nitrogen nutrition in Phalaenopsis aimed at the effects of fertilizer nitrogen concentration on plant growth. However, no reference is available on the demands of nitrogen in different stages of Phalaenopsis. Moreover, Phalaenopsis response very slowly to fertilization. According to our previous studies, it is attributed to that the thick roots and leaves may have a function of nutrient storage. Traditional nutrition research methods are thus impractical for studying the uptake and partitioning of nitrogen in Phalaenopsis. The objectives of this study are to understand the absorption and partitioning of nitrogen, and the nitrogen sources for stalk development in Phalaenopsis. We will use isotopic nitrogen (15N) as a tracer to overcome the technical difficulties mentioned above. 15Nitrogen is a stable isotope, thus an environment-friendly research tool. By feeding plants with 15N-enriched fertilizer, we can study the movement of nitrogen within plant organs. Furthermore, we will have a detailed investigation on the uptake and transportation of nitrogen during the vegetative or reproductive stages, and the roles of organs in the nitrogen sink-source relationship. The likelihood for scientific success of this project is relatively high. The results of this basic research on nitrogen uptake and partitioning are also expected to improve the fertilization management for Phalaenopsis and to benefit the industry.蝴蝶蘭氮素穩定同位素蘭花無機營養Phalaenopsisnitrogenstable isotopeorchidmineral nutrition