Lin T.-Y, BOR-LUEN CHIANGBOR-LUEN CHIANGLin T.-Y2021-07-022021-07-0219970264-410Xhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-0030834809&doi=10.1016%2fS0264-410X%2897%2900127-8&partnerID=40&md5=b7e6a4bf963293b46acb41bcbd26b5d0https://scholars.lib.ntu.edu.tw/handle/123456789/568122Recent epidemiologic data have indicated that adults are the most important reservoir that transmit pertussis to children. However conventional whole cell pertussis vaccine is contraindicated in adults and children over 7 years of age because of the unacceptably high rate of adverse reactions. The aim of this study is to evaluate the specific cellular immune responses and adverse reactions to a less reactogenic acellular pertussis vaccine in adult volunteers. Eighty healthy medical personnel in Chang Gung Children's Hospital were enrolled. Volunteers in each group received: (1) Td + full strength acellular pertussis vaccine (PT, 1 μg/0.5 ml; FHA, 4 μg/0.5 ml); (2) Td + half strength acellular pertussis vaccine; (3) Td alone. Lymphocyte phenotypic analysis, antigen-specific antibody titers, antigen-specific proliferative response and cytokine levels were evaluated before and 1 month after vaccination. Our data revealed: (I) the adverse reactions were minimal; (2) phenotypic analysis showed no non-specific activation of helper T or memory T cell after vaccination; (3) both PT and FHA-specific antibody titers increased significantly after vaccination; (4) PT antigens had a mitogenic effect on cold blood mononuclear cells and peripheral blood mononuclear cells of the adult volunteers; (5) FHA-specific T cell proliferative responses significantly increased after vaccination; (6) the cytokine production pattern showed predominant activation of Th 1 cells as reflected in increased production of γ-IFN after vaccination. Acellular pertussis vaccine can effectively induce both humoral and cellular immune response in adults.Acellular pertussis vaccine; Cytokine; Filamentous hemagglutinin; Pertussis toxin[SDGs]SDG3cytokine; hemagglutinin; pertussis vaccine; adult; anorexia nervosa; antibody titer; article; cellular immunity; clinical trial; controlled clinical trial; controlled study; drug safety; fever; helper cell; human; human experiment; immune response; injection pain; irritability; medical personnel; normal human; priority journal; randomized controlled trial; side effect; somnolence; volunteer; Adhesins, Bacterial; Adult; Aging; Antibodies, Bacterial; Antibody Specificity; Antigens, Bacterial; Cytokines; Health Personnel; Hemagglutinins; Humans; Immunity, Cellular; Leukocytes, Mononuclear; Lymphocyte Activation; Pertussis Vaccine; Phenotype; T-Lymphocytes; T-Lymphocytes, Helper-Inducer; Vaccination; Virulence Factors, Bordetella; Whooping Coughjournal article10.1016/S0264-410X(97)00127-894131022-s2.0-0030834809