2011-02-092024-05-17https://scholars.lib.ntu.edu.tw/handle/123456789/679175摘要：根據衛生署 2010 年公布的資料顯示，肝癌高居台灣男性與女性癌症死因之第一與第二位，可見肝癌防治的重要性。近年許多研究發現，牛樟芝（Antrodia cinnamomea）具有護肝、免疫調節、抑癌、抗發炎等生理活性；牛樟芝子實體萃取物在細胞與動物模式已證實其抑制肝癌的功效，然而對其活性成分與抑癌機轉尚未清楚。本研究欲以人類肝癌Hep 3B 細胞株為模式，探討牛樟芝子實體萃取物及純化物之抑癌活性及其分子機制，包括三類「eburicoic acid + dehydroeburicoic acid」、固醇類「antcin B、antcin C」及芳香族「7-hydroxy-4-methoxy-5-methyl- 1,3-benzodioxole」等。本計畫擬分兩年完成，第一年將評估牛樟芝子實體萃取物和多種純化物對肝癌細胞生長之抑制效果，並篩選主要的活性成分。於第二年深入探討其相關的抑癌機制，包括細胞週期之調控、促進細胞凋亡與壞死，尤其針對牛樟芝目前較少被探討抑癌機轉－自體吞噬（autophagy），觀察其誘導此路徑造成細胞死亡的可能性，並以大鼠初代肝細胞評估牛樟芝抗肝癌活性成分對正常細胞之影響，以期作為開發護肝保健食品之參考。<br> Abstract: The liver is a major organ in the human body for metabolic, detoxificatic and antioxidant systems. In Taiwan, 2009, cancer is a leading cause of death, and liver cancer is the second leading cause of all cancer deaths. Therefore, how to keep the liver healthy is a big issue in Taiwan. Recently, research has shown that Antrodia cinnamomea contains many active compounds which can manipulate biological activities, including liver protection, anti-cancer, anti-inflammation, and regulation of the immune system. Although studies using both cell and animal models have shown that the fruiting body of Antrodia cinnamomea has anti-cancer effects, its active compounds with anti-liver cancer activity and the exact mechanism are still unclear. In this study, the Hep 3B hepatoma cells will be used to investigate the anti-liver cancer activity of the active compounds in the fruiting body of Antrodia cinnamomea, including “eburicoic acid and dehydroeburicoic acid” belonged to triterpenoids, “antcin B and antcin C” belonged to steroids, and "7-hydroxy-4-methoxy-5-methyl-1,3-benzodioxole” belonged to aromatic compounds. In addition, the anti-liver cancer mechanisms of these compounds will be studied. This project will be completed in two parts/years. In the first part, we will investigate the anti-liver cancer activity of the fruiting body of Antrodia cinnamomea, and its various active compounds. In the second part, we will study the potential mechanisms,such as regulation of cell cycle, necrosis and apoptosis, and particularly autophagy. The rat primary hepatic cell will be used to evaluate the anti-liver-protection effects of the compounds of Antrodia cinnamomea on normal cells. Hopefully, the main active compounds with anti-liver cancer activity from the fruiting body of Antrodia cinnamome will be found and used as an important reference for health food development.肝癌牛樟芝子實體三類固醇類芳香族細胞壞死細胞凋亡自體吞噬liver cancerfruiting body of Antrodia cinnamomeatriterpenoidssteroidsaromatic compounds,牛樟芝子實體中抗肝癌活性成分之探討