2020-06-012024-05-18https://scholars.lib.ntu.edu.tw/handle/123456789/709338摘要：本計畫目的為應用新型微脂體奈米材料包覆鈣或磷離子溶液，運用於齒內治療，解決牙本質小管暴露相關衍生之疾病。從第一、二年成果得知，磷酸二氫鉀可被微脂體成功包裹，且在中性環境下能快速通過牙本質小管，而氯化鈣經微脂體或經聚乙二醇 (Polyethylene glycol, mPEG) 修飾之微脂體包裹會形成團聚效應，導致氯化鈣無法被穩定包覆。欲使氯化鈣能被穩定包覆，以 β-環糊精 (β-cyclodextrin, β-CD) 與鈣離子形成錯合物後，再以磷脂醯膽鹼 (L-α-phosphatidylcholine, PC)、磷脂絲胺酸 (Phosphatidylserine, PS) 微脂體包覆，發現經滲透牙本質試片可觀察到牙本質小管內有大量的結晶沉澱，顯示其能順利滲透試片，進而封填牙本質小管。第三年將依據第一、二年篩選出組別，分別為 PC、PS 微脂體搭載含鈣環糊精，以及對照組孔洞鈣矽材料CCMS (研究團隊先前之研發)三組，以進行牙齒敏感症、活髓治療及根管內置藥之李宋豬齒內治療動物模型，進而分析比較其封閉牙本質小管與細微側根管之能力。經由組織切片觀察組織發炎狀態、電腦斷層掃描影像處理 (Micro Computed Tomography Imaging System, &micro;-CT) 分析硬組織的生成，以掃描式電子顯微鏡 (Scanning Electron Microscope, SEM) 觀察滲透牙本質小管及沉澱封閉牙本質小管之能力，並探討微脂體的藥物釋放及牙本質小管結晶封填之機制。<br> Abstract: This study aims to apply novel materials such as liposomes to encapsulate calcium or phosphate buffer solutions to treat exposed dentinal tubules and their related diseases. The results of our first-year and second-year studies showed that K2HPO4 could be encapsulated by liposomes and passed through dentinal tubules rapidly in the neutral environment. Furthermore, liposomes were modified by polyethylene glycol (mPEG) to increase the stability of its structure. However, when mPEG-modified liposomes encapsulated CaCl2 would form the aggregates, which could not pass through dentinal tubules, and the CaCl2 were also not encapsulated by liposomes stably. In order to encapsulate CaCl2 stably by liposomes, β-cyclodextrin (β-CD) was added to form a complex and encapsulate it with L-α-phosphatidylcholine (PC) and phosphatidylserine (PS). The results of the dentin disc penetration test showed that most dentinal tubules had a large number of precipitations accumulated, which could block dentinal tubules. In the third-year study, PC and PS liposomes will encapsulate β-CD to compare with CaCO3@mesoporous silica (CCMS, previously developed by our research group) as the control group to perform animal studies for dentin hypersensitivity, vital pulp therapy, and intra-canal medication. The ability to block dentinal tubules and the lateral canal of these materials will be compared and analyzed. Histologic section and Micro Computed Tomography Imaging System (&micro;-CT) will be performed to observe the inflammation condition of the tissue and the formation of the hard tissue. The ability of liposomes penetrating dentin disc and blocking dentinal tubules will be observed by Scanning Electron Microscope (SEM). The mechanisms of blocking dentinal tubules and the drug release of liposomes with ionic solutions will also be investigated.微脂體牙本質小管牙齒敏感症活髓治療根管內置藥LiposomeDentinal tubulesDentin hypersensitivityVital pulp therapyIntra-canal medication