Tai Y.-HLo S.-CMontagne KTsai P.-CLiao C.-CWang S.-HChin I.-SXing DHo Y.-LNIEN-TSU HUANGWei P.-KDelaunay J.-J.2022-04-252022-04-25202109565663https://www.scopus.com/inward/record.uri?eid=2-s2.0-85108868690&doi=10.1016%2fj.bios.2021.113463&partnerID=40&md5=65ea296ee57cf8074f109efa0fa8275dhttps://scholars.lib.ntu.edu.tw/handle/123456789/607262An osmium-coated lensed fiber (OLF) probe combined with a silver-coated black silicon (SBS) substrate was used to generate a dielectrophoretic (DEP) force that traps bacteria and enables Raman signal detection from bacteria. The lensed fiber coated with a 2-nm osmium layer was used as an electrode for the DEP force and also as a lens to excite Raman signals. The black silicon coated with a 150-nm silver layer was used both as the surface-enhanced Raman scattering (SERS) substrate and the counter electrode. The enhanced Raman signal was collected by the same OLF probe and further analyzed with a spectrometer. For Raman measurements, a drop of bacterial suspension was placed between the OLF probe and the SBS substrate. By controlling the frequency of an AC voltage on the OLF probe and SBS substrate, a DEP force at 1 MHz concentrated bacteria on the SBS surface and removed the unbound micro-objects in the solution at 1 kHz. A bacteria concentration of 6 × 104 CFU/mL (colony forming units per mL) could be identified in less than 15 min, using a volume of only 1 μL, by recording the variation of the Raman peak at 740 cm?1. ? 2021 Elsevier B.V.Bacteria detectionDielectrophoretic forceLens fiberRaman enhancementDielectric devicesElectrodesProbesSiliconSilverSubstratesBlack siliconCoated blacksDielectrophoretic forcesFibre probeLens-fibreLensed fibersRaman signalSilicon substratesBacteriasilicon derivativesiliconArticlebacteriumbacterium detectionchemical analysiscoating (procedure)colony forming unitconcentration (parameter)controlled studynonhumanRaman spectrometryAfrican Americangenetic procedureshumanAfrican AmericansBiosensing TechniquesHumansSpectrum Analysis, Raman[SDGs]SDG3journal article10.1016/j.bios.2021.11346334198171341981712-s2.0-85108868690